Bioengineering of bacterial magnetic particles and its application to estrogen receptor-ligand binding assay

Tomoko Yoshino*, Chihiro Kaji, Tadashi Matsunaga

*この研究の対応する著者

研究成果: Conference contribution

2 被引用数 (Scopus)

抄録

Magnetic particles are used for various biomedical applications because they are easy to both handle and separate from biological samples. Nano-sized bacterial magnetic particles (BacMPs) that display the human estrogen receptor ligand binding domain (ERLBD) on their surfaces were successfully produced by the magnetotactic bacterium, Magnetospirillum inagneticum AMB-1. A receptor assay for endocrine-disrupting chemicals using ERLBD- displaying BacMPs was developed. A BacMP membrane-specific protein, Mmsl6 or Mmsl3, was used as an anchor protein to localize the ERLBD on the surfaces of BacMPs. ERLBD- BacMP complexes were assayed for competitive binding of alkaline phosphatase- conjugated 17(3-estradiol (ALP-E2). Inhibition curve of ALP-E2 to the powerful antagonist, tamoxifen was generated by measuring decreases in luminescence intensity that resulted from the enzymatic reaction of alkaline phosphatase. The overall simplicity of this receptor-binding assay results in a method that can be easily adapted to a high-throughput format.

本文言語English
ホスト出版物のタイトルFrom Biological Materials to Biomimetic Material Synthesis
ページ13-18
ページ数6
1094
出版ステータスPublished - 2008
外部発表はい
イベント2008 MRS Spring Meeting - San Francisco, CA
継続期間: 2008 3 242008 3 28

Other

Other2008 MRS Spring Meeting
CitySan Francisco, CA
Period08/3/2408/3/28

ASJC Scopus subject areas

  • 材料科学(全般)
  • 凝縮系物理学
  • 機械工学
  • 材料力学

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