Biohybrid Actuators Based on Skeletal Muscle-Powered Microgrooved Ultrathin Films Consisting of Poly(styrene- block-butadiene- block-styrene)

Arihiro Hasebe, Yoshitaka Suematsu, Shinji Takeoka, Tommaso Mazzocchi, Lorenzo Vannozzi, Leonardo Ricotti, Toshinori Fujie

研究成果: Article

抄録

This paper describes a biohybrid actuator consisting of a microgrooved thin film, powered by contractile, aligned skeletal muscle cells. The system was made of a thermoplastic elastomer [SBS, poly(styrene-block-butadiene-block-styrene)]. We prepared SBS thin films with different thicknesses (0.5-11.7 μm) and Young's moduli (46.7-68.6 MPa) to vary their flexural rigidity. The microgrooves on the SBS thin film resembled the microstructure of the extracellular matrix of muscle and facilitated the alignment and differentiation of skeletal muscle cells. Electrical stimulation was applied to self-standing biohybrid thin films to trigger their contraction, enabled by the low flexural rigidity of the SBS thin film. Finite element model simulations were also examined to predict their contractile behavior. We achieved the prediction of displacements, which were rather close to the actual values of the SBS thin film: the discrepancy was <5% on the X axis. These results pave the way for in silico prediction of the contractile capabilities of elastomeric thin films. This study highlights the potential of microgrooved SBS thin films as ultraflexible platforms for biohybrid machines.

元の言語English
ジャーナルACS Biomaterials Science and Engineering
DOI
出版物ステータスPublished - 2019 1 1

Fingerprint

Styrene
Ultrathin films
Butadiene
Muscle
Actuators
Thin films
Rigidity
1,3-butadiene
Cells
Thermoplastic elastomers
Elastic moduli

Keywords

    ASJC Scopus subject areas

    • Biomaterials
    • Biomedical Engineering

    これを引用

    Biohybrid Actuators Based on Skeletal Muscle-Powered Microgrooved Ultrathin Films Consisting of Poly(styrene- block-butadiene- block-styrene). / Hasebe, Arihiro; Suematsu, Yoshitaka; Takeoka, Shinji; Mazzocchi, Tommaso; Vannozzi, Lorenzo; Ricotti, Leonardo; Fujie, Toshinori.

    :: ACS Biomaterials Science and Engineering, 01.01.2019.

    研究成果: Article

    Hasebe, Arihiro ; Suematsu, Yoshitaka ; Takeoka, Shinji ; Mazzocchi, Tommaso ; Vannozzi, Lorenzo ; Ricotti, Leonardo ; Fujie, Toshinori. / Biohybrid Actuators Based on Skeletal Muscle-Powered Microgrooved Ultrathin Films Consisting of Poly(styrene- block-butadiene- block-styrene). :: ACS Biomaterials Science and Engineering. 2019.
    @article{f389c1ebb96c4361b279137d5b4aa089,
    title = "Biohybrid Actuators Based on Skeletal Muscle-Powered Microgrooved Ultrathin Films Consisting of Poly(styrene- block-butadiene- block-styrene)",
    abstract = "This paper describes a biohybrid actuator consisting of a microgrooved thin film, powered by contractile, aligned skeletal muscle cells. The system was made of a thermoplastic elastomer [SBS, poly(styrene-block-butadiene-block-styrene)]. We prepared SBS thin films with different thicknesses (0.5-11.7 μm) and Young's moduli (46.7-68.6 MPa) to vary their flexural rigidity. The microgrooves on the SBS thin film resembled the microstructure of the extracellular matrix of muscle and facilitated the alignment and differentiation of skeletal muscle cells. Electrical stimulation was applied to self-standing biohybrid thin films to trigger their contraction, enabled by the low flexural rigidity of the SBS thin film. Finite element model simulations were also examined to predict their contractile behavior. We achieved the prediction of displacements, which were rather close to the actual values of the SBS thin film: the discrepancy was <5{\%} on the X axis. These results pave the way for in silico prediction of the contractile capabilities of elastomeric thin films. This study highlights the potential of microgrooved SBS thin films as ultraflexible platforms for biohybrid machines.",
    keywords = "biohybrid actuator, flexural rigidity, microgrooves, organic machine, polymeric thin films, SBS, skeletal muscle",
    author = "Arihiro Hasebe and Yoshitaka Suematsu and Shinji Takeoka and Tommaso Mazzocchi and Lorenzo Vannozzi and Leonardo Ricotti and Toshinori Fujie",
    year = "2019",
    month = "1",
    day = "1",
    doi = "10.1021/acsbiomaterials.8b01550",
    language = "English",
    journal = "ACS Biomaterials Science and Engineering",
    issn = "2373-9878",
    publisher = "American Chemical Society",

    }

    TY - JOUR

    T1 - Biohybrid Actuators Based on Skeletal Muscle-Powered Microgrooved Ultrathin Films Consisting of Poly(styrene- block-butadiene- block-styrene)

    AU - Hasebe, Arihiro

    AU - Suematsu, Yoshitaka

    AU - Takeoka, Shinji

    AU - Mazzocchi, Tommaso

    AU - Vannozzi, Lorenzo

    AU - Ricotti, Leonardo

    AU - Fujie, Toshinori

    PY - 2019/1/1

    Y1 - 2019/1/1

    N2 - This paper describes a biohybrid actuator consisting of a microgrooved thin film, powered by contractile, aligned skeletal muscle cells. The system was made of a thermoplastic elastomer [SBS, poly(styrene-block-butadiene-block-styrene)]. We prepared SBS thin films with different thicknesses (0.5-11.7 μm) and Young's moduli (46.7-68.6 MPa) to vary their flexural rigidity. The microgrooves on the SBS thin film resembled the microstructure of the extracellular matrix of muscle and facilitated the alignment and differentiation of skeletal muscle cells. Electrical stimulation was applied to self-standing biohybrid thin films to trigger their contraction, enabled by the low flexural rigidity of the SBS thin film. Finite element model simulations were also examined to predict their contractile behavior. We achieved the prediction of displacements, which were rather close to the actual values of the SBS thin film: the discrepancy was <5% on the X axis. These results pave the way for in silico prediction of the contractile capabilities of elastomeric thin films. This study highlights the potential of microgrooved SBS thin films as ultraflexible platforms for biohybrid machines.

    AB - This paper describes a biohybrid actuator consisting of a microgrooved thin film, powered by contractile, aligned skeletal muscle cells. The system was made of a thermoplastic elastomer [SBS, poly(styrene-block-butadiene-block-styrene)]. We prepared SBS thin films with different thicknesses (0.5-11.7 μm) and Young's moduli (46.7-68.6 MPa) to vary their flexural rigidity. The microgrooves on the SBS thin film resembled the microstructure of the extracellular matrix of muscle and facilitated the alignment and differentiation of skeletal muscle cells. Electrical stimulation was applied to self-standing biohybrid thin films to trigger their contraction, enabled by the low flexural rigidity of the SBS thin film. Finite element model simulations were also examined to predict their contractile behavior. We achieved the prediction of displacements, which were rather close to the actual values of the SBS thin film: the discrepancy was <5% on the X axis. These results pave the way for in silico prediction of the contractile capabilities of elastomeric thin films. This study highlights the potential of microgrooved SBS thin films as ultraflexible platforms for biohybrid machines.

    KW - biohybrid actuator

    KW - flexural rigidity

    KW - microgrooves

    KW - organic machine

    KW - polymeric thin films

    KW - SBS

    KW - skeletal muscle

    UR - http://www.scopus.com/inward/record.url?scp=85064180148&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=85064180148&partnerID=8YFLogxK

    U2 - 10.1021/acsbiomaterials.8b01550

    DO - 10.1021/acsbiomaterials.8b01550

    M3 - Article

    JO - ACS Biomaterials Science and Engineering

    JF - ACS Biomaterials Science and Engineering

    SN - 2373-9878

    ER -