Bivalent interaction of the PZP domain of BRPF1 with the nucleosome impacts chromatin dynamics and acetylation

Brianna J. Klein, Uma M. Muthurajan, Marie Eve Lalonde, Matthew D. Gibson, Forest H. Andrews, Maggie Hepler, Shinichi Machida, Kezhi Yan, Hitoshi Kurumizaka, Michael G. Poirier, Jacques Côté, Karolin Luger*, Tatiana G. Kutateladze

*この研究の対応する著者

    研究成果: Article査読

    33 被引用数 (Scopus)

    抄録

    BRPF1 (bromodomain PHD finger 1) is a core subunit of the MOZ histone acetyltransferase (HAT) complex, critical for normal developmental programs and implicated in acute leukemias. BRPF1 contains a unique assembly of zinc fingers, termed a PZP domain, the physiological role of which remains unclear. Here, we elucidate the structure-function relationship of this novel epigenetic reader and detail the biological and mechanistic consequences of its interaction with nucleosomes. PZP has a globular architecture and forms a 2:1 stoichiometry complex with the nucleosome, bivalently interacting with histone H3 and DNA. This binding impacts the nucleosome dynamics, shifting the DNA unwrapping/rewrapping equilibrium toward the unwrapped state and increasing DNA accessibility. We demonstrate that the DNA-binding function of the BRPF1 PZP domain is required for the MOZ-BRPF1-ING5-hEaf6 HAT complex to be recruited to chromatin and to acetylate nucleosomal histones. Our findings reveal a novel link between chromatin dynamics and MOZ-mediated acetylation.

    本文言語English
    ページ(範囲)472-484
    ページ数13
    ジャーナルNucleic Acids Research
    44
    1
    DOI
    出版ステータスPublished - 2016 1月 8

    ASJC Scopus subject areas

    • 遺伝学

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