Brain cytokine and chemokine mRNA expression in mice induced by intranasal instillation with ultrafine carbon black

Tin-Tin-Win-Shwe*, Shoji Yamamoto, Sohel Ahmed, Masaki Kakeyama, Takahiro Kobayashi, Hidekazu Fujimaki

*この研究の対応する著者

研究成果: Article査読

103 被引用数 (Scopus)

抄録

Ambient air ultrafine particles (UFPs) have gained enormous attention to many researchers with recent evidence showing them to have more hazardous effects on human health than larger ambient particles. Studies focusing the possibility of effects on brain are quite limited. To examine the effect of ultrafine carbon black (ufCB) on mice brain, we instilled 125 μg of 14 nm or 95 nm CB into the nostrils of 8-week-old male BALB/c mice, once a week for 4 weeks. Four hours after the last instillation, we collected olfactory bulb and hippocampus and detected the expression of cytokine and chemokine mRNA by quantitative real-time PCR method. In this study, we found the induction of proinflammatory cytokines (interleukin-1β and tumor necrosis factor-α and chemokines (monocyte chemoattractant protein-1/CCL2, macrophage inflammatory protein-1α/CCL3), and monokine induced interferon-gamma/CXC chemokine ligand (CXCL9) mRNA in brain olfactory bulb, not in the hippocampus of mice instilled with 14 nm ufCB intranasally. We suggest that the intranasal instillation of ufCB may influence the brain immune function depending on their size. To our knowledge, this is the first study to demonstrate region-specific brain cytokine and chemokine mRNA-induction in mice triggered by intranasal instillation of specific-sized ufCB, in a physiologically relevant condition.

本文言語English
ページ(範囲)153-160
ページ数8
ジャーナルToxicology Letters
163
2
DOI
出版ステータスPublished - 2006 5 25
外部発表はい

ASJC Scopus subject areas

  • 毒物学

フィンガープリント

「Brain cytokine and chemokine mRNA expression in mice induced by intranasal instillation with ultrafine carbon black」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル