Budding yeast Cdc5 phosphorylates Net1 and assists Cdc14 release from the nucleolus

Satoshi Yoshida, Akio Toh-e*

*この研究の対応する著者

研究成果: Article査読

52 被引用数 (Scopus)

抄録

Polo-like kinase Cdc5 and Cdc14 phosphatase are essential for mitotic exit in budding yeast. Cdc14 sequestered in the nucleolus by forming a complex with Net1, a nucleolar inhibitor of Cdc14, is activated after the release from the nucleolus and Cdc5 is essential for this release. Here we show that Cdc5 affects the phosphorylation state of Net1. Tab6 is a dominant active form of Cdc14. We found that Tab6 was released from the nucleolus of cdc5 mutant cells in a cell cycle dependent manner and that the release of Tab6 (or Cdc14) was not sufficient for the cdc5 mutant to grow at a higher temperature. Altogether, we propose that Cdc5 acts to reduce affinity between Cdc14 and Net1 and that the timing of Cdc14 release is independent of Cdc5. We also provide evidence that the critical function of Cdc5, other than Cdc14 liberation, exists in late mitotic events.

本文言語English
ページ(範囲)687-691
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
294
3
DOI
出版ステータスPublished - 2002
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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