Calcium-induced mechanical change in the neck domain alters the activity of plant myosin XI

Motoki Tominaga, Hiroaki Kojima, Etsuo Yokota, Rinna Nakamori, Michael Anson, Teruo Shimmen, Kazuhiro Oiwa

研究成果: Article

26 引用 (Scopus)

抄録

Plant myosin XI functions as a motor that generates cytoplasmic streaming in plant cells. Although cytoplasmic streaming is known to be regulated by intracellular Ca2+ concentration, the molecular mechanism underlying this control is not fully understood. Here, we investigated the mechanism of regulation of myosin XI by Ca2+ at the molecular level. Actin filaments were easily detached from myosin XI in an in vitro motility assay at high Ca2+ concentration (pCa 4) concomitant with the detachment of calmodulin light chains from the neck domains. Electron microscopic observations showed that myosin XI at pCa 4 shortened the neck domain by 30%. Single-molecule analysis revealed that the step size of myosin XI at pCa 4 was shortened to 27 nm under low load and to 22 nm under high load compared with 35 nm independent of the load for intact myosin XI. These results indicate that modulation of the mechanical properties of the neck domain is a key factor for achieving the Ca2+-induced regulation of cytoplasmic streaming.

元の言語English
ページ(範囲)30711-30718
ページ数8
ジャーナルJournal of Biological Chemistry
287
発行部数36
DOI
出版物ステータスPublished - 2012 8 31
外部発表Yes

Fingerprint

Myosins
Neck
Cytoplasmic Streaming
Calcium
Plant Cells
Calmodulin
Actin Cytoskeleton
Actins
Assays
Modulation
Electrons
Light
Mechanical properties
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

これを引用

Calcium-induced mechanical change in the neck domain alters the activity of plant myosin XI. / Tominaga, Motoki; Kojima, Hiroaki; Yokota, Etsuo; Nakamori, Rinna; Anson, Michael; Shimmen, Teruo; Oiwa, Kazuhiro.

:: Journal of Biological Chemistry, 巻 287, 番号 36, 31.08.2012, p. 30711-30718.

研究成果: Article

Tominaga, M, Kojima, H, Yokota, E, Nakamori, R, Anson, M, Shimmen, T & Oiwa, K 2012, 'Calcium-induced mechanical change in the neck domain alters the activity of plant myosin XI', Journal of Biological Chemistry, 巻. 287, 番号 36, pp. 30711-30718. https://doi.org/10.1074/jbc.M112.346668
Tominaga, Motoki ; Kojima, Hiroaki ; Yokota, Etsuo ; Nakamori, Rinna ; Anson, Michael ; Shimmen, Teruo ; Oiwa, Kazuhiro. / Calcium-induced mechanical change in the neck domain alters the activity of plant myosin XI. :: Journal of Biological Chemistry. 2012 ; 巻 287, 番号 36. pp. 30711-30718.
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abstract = "Plant myosin XI functions as a motor that generates cytoplasmic streaming in plant cells. Although cytoplasmic streaming is known to be regulated by intracellular Ca2+ concentration, the molecular mechanism underlying this control is not fully understood. Here, we investigated the mechanism of regulation of myosin XI by Ca2+ at the molecular level. Actin filaments were easily detached from myosin XI in an in vitro motility assay at high Ca2+ concentration (pCa 4) concomitant with the detachment of calmodulin light chains from the neck domains. Electron microscopic observations showed that myosin XI at pCa 4 shortened the neck domain by 30{\%}. Single-molecule analysis revealed that the step size of myosin XI at pCa 4 was shortened to 27 nm under low load and to 22 nm under high load compared with 35 nm independent of the load for intact myosin XI. These results indicate that modulation of the mechanical properties of the neck domain is a key factor for achieving the Ca2+-induced regulation of cytoplasmic streaming.",
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AU - Tominaga, Motoki

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AU - Yokota, Etsuo

AU - Nakamori, Rinna

AU - Anson, Michael

AU - Shimmen, Teruo

AU - Oiwa, Kazuhiro

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