Capsid protein oxidation in feline calicivirus using an electrochemical inactivation treatment

Nozomi Shionoiri, Osamu Nogariya, Masayoshi Tanaka, Tadashi Matsunaga, Tsuyoshi Tanaka*

*この研究の対応する著者

研究成果: Article査読

12 被引用数 (Scopus)

抄録

Pathogenic viral infections are an international public health concern, and viral disinfection has received increasing attention. Electrochemical treatment has been used for treatment of water contaminated by bacteria for several decades, and although in recent years several reports have investigated viral inactivation kinetics, the mode of action of viral inactivation by electrochemical treatment remains unclear. Here, we demonstrated the inactivation of feline calicivirus (FCV), a surrogate for human noroviruses, by electrochemical treatment in a developed flow-cell equipped with a screen-printed electrode. The viral infectivity titer was reduced by over 5 orders of magnitude after 15 min of treatment at 0.9V vs. Ag/AgCl. Proteomic study of electrochemically inactivated virus revealed oxidation of peptides located in the viral particles; oxidation was not observed in the non-treated sample. Furthermore, transmission electron microscopy revealed that viral particles in the treated sample had irregular structures. These results suggest that electrochemical treatment inactivates FCV via oxidation of peptides in the structural region, causing structural deformation of virus particles. This first report of viral protein damage through electrochemical treatment will contribute to broadening the understanding of viral inactivation mechanisms.

本文言語English
ページ(範囲)410-415
ページ数6
ジャーナルJournal of Hazardous Materials
283
DOI
出版ステータスPublished - 2015 1月 1
外部発表はい

ASJC Scopus subject areas

  • 環境工学
  • 環境化学
  • 医学(全般)
  • 廃棄物管理と処理
  • 汚染
  • 健康、毒物学および変異誘発

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