抄録
Bacterial magnetic particles (BMPs) were modified with 3-[2-(2- aminoethylamino)-ethylamino]-propyltrimethoxysilane (AEEA) to produce a dense amine surface. Modification of BMPs in a toluene solution resulted in an increased amine yield, and approximately 11.3 × 104 surface amines were detected on a single particle. The modified BMPs were capable of efficient electrostatic capture of DNA. The maximum amount of DNA captured on 10 μg of aminosilane-modified BMPs was 600 ng. A 10 mM phosphate buffer effectively released the captured DNA. This efficiency was dramatically enhanced by incubation at 80°C and DNA recovery from aminosilane-modified BMPs approached 95%. DNA extraction from whole blood using these modified BMPs, followed by PCR, was successfully performed. Furthermore, automated single nucleotide polymorphism (SNP) detection of the aldehyde dehydrogenase 2 (ALDH2) was demonstrated.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 862-868 |
| ページ数 | 7 |
| ジャーナル | Biotechnology and Bioengineering |
| 巻 | 94 |
| 発行部数 | 5 |
| DOI | |
| 出版物ステータス | Published - 2006 8 5 |
| 外部発表 | Yes |
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ASJC Scopus subject areas
- Biotechnology
- Microbiology
これを引用
Capture and release of DNA using aminosilane-modified bacterial magnetic particles for automated detection system of single nucleotide polymorphisms. / Nakagawa, Takahito; Hashimoto, Reisuke; Maruyama, Kohei; Tanaka, Tsuyoshi; Takeyama, Haruko; Matsunaga, Tadashi.
：: Biotechnology and Bioengineering, 巻 94, 番号 5, 05.08.2006, p. 862-868.研究成果: Article
}
TY - JOUR
T1 - Capture and release of DNA using aminosilane-modified bacterial magnetic particles for automated detection system of single nucleotide polymorphisms
AU - Nakagawa, Takahito
AU - Hashimoto, Reisuke
AU - Maruyama, Kohei
AU - Tanaka, Tsuyoshi
AU - Takeyama, Haruko
AU - Matsunaga, Tadashi
PY - 2006/8/5
Y1 - 2006/8/5
N2 - Bacterial magnetic particles (BMPs) were modified with 3-[2-(2- aminoethylamino)-ethylamino]-propyltrimethoxysilane (AEEA) to produce a dense amine surface. Modification of BMPs in a toluene solution resulted in an increased amine yield, and approximately 11.3 × 104 surface amines were detected on a single particle. The modified BMPs were capable of efficient electrostatic capture of DNA. The maximum amount of DNA captured on 10 μg of aminosilane-modified BMPs was 600 ng. A 10 mM phosphate buffer effectively released the captured DNA. This efficiency was dramatically enhanced by incubation at 80°C and DNA recovery from aminosilane-modified BMPs approached 95%. DNA extraction from whole blood using these modified BMPs, followed by PCR, was successfully performed. Furthermore, automated single nucleotide polymorphism (SNP) detection of the aldehyde dehydrogenase 2 (ALDH2) was demonstrated.
AB - Bacterial magnetic particles (BMPs) were modified with 3-[2-(2- aminoethylamino)-ethylamino]-propyltrimethoxysilane (AEEA) to produce a dense amine surface. Modification of BMPs in a toluene solution resulted in an increased amine yield, and approximately 11.3 × 104 surface amines were detected on a single particle. The modified BMPs were capable of efficient electrostatic capture of DNA. The maximum amount of DNA captured on 10 μg of aminosilane-modified BMPs was 600 ng. A 10 mM phosphate buffer effectively released the captured DNA. This efficiency was dramatically enhanced by incubation at 80°C and DNA recovery from aminosilane-modified BMPs approached 95%. DNA extraction from whole blood using these modified BMPs, followed by PCR, was successfully performed. Furthermore, automated single nucleotide polymorphism (SNP) detection of the aldehyde dehydrogenase 2 (ALDH2) was demonstrated.
KW - Aminosilane compound
KW - Automated system
KW - Bacterial magnetic particles (BMPs)
KW - DNA capture
KW - Phosphate
KW - Single nucleotide polymorphism (SNP)
UR - http://www.scopus.com/inward/record.url?scp=33746887470&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33746887470&partnerID=8YFLogxK
U2 - 10.1002/bit.20904
DO - 10.1002/bit.20904
M3 - Article
C2 - 16523525
AN - SCOPUS:33746887470
VL - 94
SP - 862
EP - 868
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
SN - 0006-3592
IS - 5
ER -