抄録
Bacterial magnetic particles (BMPs) were modified with 3-[2-(2- aminoethylamino)-ethylamino]-propyltrimethoxysilane (AEEA) to produce a dense amine surface. Modification of BMPs in a toluene solution resulted in an increased amine yield, and approximately 11.3 × 104 surface amines were detected on a single particle. The modified BMPs were capable of efficient electrostatic capture of DNA. The maximum amount of DNA captured on 10 μg of aminosilane-modified BMPs was 600 ng. A 10 mM phosphate buffer effectively released the captured DNA. This efficiency was dramatically enhanced by incubation at 80°C and DNA recovery from aminosilane-modified BMPs approached 95%. DNA extraction from whole blood using these modified BMPs, followed by PCR, was successfully performed. Furthermore, automated single nucleotide polymorphism (SNP) detection of the aldehyde dehydrogenase 2 (ALDH2) was demonstrated.
本文言語 | English |
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ページ(範囲) | 862-868 |
ページ数 | 7 |
ジャーナル | Biotechnology and bioengineering |
巻 | 94 |
号 | 5 |
DOI | |
出版ステータス | Published - 2006 8月 5 |
外部発表 | はい |
ASJC Scopus subject areas
- バイオテクノロジー
- バイオエンジニアリング
- 応用微生物学とバイオテクノロジー