Carbonylated proteins in aging and exercise: Immunoblot approaches

S. Goto*, A. Nakamura, Z. Radak, H. Nakamoto, R. Takahashi, K. Yasuda, Y. Sakurai, N. Ishii

*この研究の対応する著者

研究成果: Article査読

91 被引用数 (Scopus)

抄録

Protein carbonyls were studied in aging and exercise by immunoblot followed by one- or two-dimensional polyacrylamide gel electrophoresis using antibodies against 2,4-dinitrophenylhydrazones. Proteins of rat kidneys exhibited significant age-related increase in the amount of carbonyl while those of the brain and liver did not. Major carbonylated proteins in the kidney included serum albumin. In nematodes in which protein carbonyls increased with age, one of the carbonylated proteins was identified as vitellogenin, an egg-yolk protein. A possible biological significance of this protein present in abundance even after egg-laying stages is discussed in terms of protection against oxidative stress. Exhaustive exercise induced significant increase in the carbonylation of selected but unidentified proteins in the lung. This oxidative stress might be caused by xanthine oxidase in this tissue and hypoxanthine derived from ATP-depleted muscles. Exercise at high altitude caused higher carbonylation of the skeletal muscle proteins, most notably a protein likely to be actin, than that at sea level but no significant difference was observed in lipid peroxidation. These studies emphasize the value of immunoblot analysis of tissue protein carbonyls in a variety of situations where oxidative stress is likely involved.

本文言語English
ページ(範囲)245-253
ページ数9
ジャーナルMechanisms of Ageing and Development
107
3
DOI
出版ステータスPublished - 1999 3月 15
外部発表はい

ASJC Scopus subject areas

  • 加齢科学
  • 発生生物学

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