Casein kinase 1 family regulates PRR5 and TOC1 in the Arabidopsis circadian clock

Takahiro N. Uehara, Yoshiyuki Mizutani, Keiko Kuwata, Tsuyoshi Hirota, Ayato Sato, Junya Mizoi, Saori Takao, Hiromi Matsuo, Takamasa Suzuki, Shogo Ito, Ami N. Saito, Taeko Nishiwaki-Ohkawa, Kazuko Yamaguchi-Shinozaki, Takashi Yoshimura, Steve A. Kay, Kenichiro Itami, Toshinori Kinoshita, Junichiro Yamaguchi, Norihito Nakamichi

研究成果: Article

抄録

The circadian clock provides organisms with the ability to adapt to daily and seasonal cycles. Eukaryotic clocks mostly rely on lineage-specific transcriptional-translational feedback loops (TTFLs). Posttranslational modifications are also crucial for clock functions in fungi and animals, but the posttranslational modifications that affect the plant clock are less understood. Here, using chemical biology strategies, we show that the Arabidopsis CASEIN KINASE 1 LIKE (CKL) family is involved in posttranslational modification in the plant clock. Chemical screening demonstrated that an animal CDC7/CDK9 inhibitor, PHA767491, lengthens the Arabidopsis circadian period. Affinity proteomics using a chemical probe revealed that PHA767491 binds to and inhibits multiple CKL proteins, rather than CDC7/CDK9 homologs. Simultaneous knockdown of Arabidopsis CKL-encoding genes lengthened the circadian period. CKL4 phosphorylated transcriptional repressors PSEUDO-RESPONSE REGULATOR 5 (PRR5) and TIMING OF CAB EXPRESSION 1 (TOC1) in the TTFL. PHA767491 treatment resulted in accumulation of PRR5 and TOC1, accompanied by decreasing expression of PRR5- and TOC1-target genes. A prr5 toc1 double mutant was hyposensitive to PHA767491-induced period lengthening. Together, our results reveal posttranslational modification of transcriptional repressors in plant clock TTFL by CK1 family proteins, which also modulate nonplant circadian clocks.

元の言語English
ページ(範囲)11528-11536
ページ数9
ジャーナルProceedings of the National Academy of Sciences of the United States of America
166
発行部数23
DOI
出版物ステータスPublished - 2019 1 1

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Casein Kinase I
Circadian Clocks
Post Translational Protein Processing
Arabidopsis
Proteomics
Genes
Proteins
Fungi
cellulose acetate-butyrate
PHA 767491

ASJC Scopus subject areas

  • General

これを引用

Casein kinase 1 family regulates PRR5 and TOC1 in the Arabidopsis circadian clock. / Uehara, Takahiro N.; Mizutani, Yoshiyuki; Kuwata, Keiko; Hirota, Tsuyoshi; Sato, Ayato; Mizoi, Junya; Takao, Saori; Matsuo, Hiromi; Suzuki, Takamasa; Ito, Shogo; Saito, Ami N.; Nishiwaki-Ohkawa, Taeko; Yamaguchi-Shinozaki, Kazuko; Yoshimura, Takashi; Kay, Steve A.; Itami, Kenichiro; Kinoshita, Toshinori; Yamaguchi, Junichiro; Nakamichi, Norihito.

:: Proceedings of the National Academy of Sciences of the United States of America, 巻 166, 番号 23, 01.01.2019, p. 11528-11536.

研究成果: Article

Uehara, TN, Mizutani, Y, Kuwata, K, Hirota, T, Sato, A, Mizoi, J, Takao, S, Matsuo, H, Suzuki, T, Ito, S, Saito, AN, Nishiwaki-Ohkawa, T, Yamaguchi-Shinozaki, K, Yoshimura, T, Kay, SA, Itami, K, Kinoshita, T, Yamaguchi, J & Nakamichi, N 2019, 'Casein kinase 1 family regulates PRR5 and TOC1 in the Arabidopsis circadian clock', Proceedings of the National Academy of Sciences of the United States of America, 巻. 166, 番号 23, pp. 11528-11536. https://doi.org/10.1073/pnas.1903357116
Uehara, Takahiro N. ; Mizutani, Yoshiyuki ; Kuwata, Keiko ; Hirota, Tsuyoshi ; Sato, Ayato ; Mizoi, Junya ; Takao, Saori ; Matsuo, Hiromi ; Suzuki, Takamasa ; Ito, Shogo ; Saito, Ami N. ; Nishiwaki-Ohkawa, Taeko ; Yamaguchi-Shinozaki, Kazuko ; Yoshimura, Takashi ; Kay, Steve A. ; Itami, Kenichiro ; Kinoshita, Toshinori ; Yamaguchi, Junichiro ; Nakamichi, Norihito. / Casein kinase 1 family regulates PRR5 and TOC1 in the Arabidopsis circadian clock. :: Proceedings of the National Academy of Sciences of the United States of America. 2019 ; 巻 166, 番号 23. pp. 11528-11536.
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abstract = "The circadian clock provides organisms with the ability to adapt to daily and seasonal cycles. Eukaryotic clocks mostly rely on lineage-specific transcriptional-translational feedback loops (TTFLs). Posttranslational modifications are also crucial for clock functions in fungi and animals, but the posttranslational modifications that affect the plant clock are less understood. Here, using chemical biology strategies, we show that the Arabidopsis CASEIN KINASE 1 LIKE (CKL) family is involved in posttranslational modification in the plant clock. Chemical screening demonstrated that an animal CDC7/CDK9 inhibitor, PHA767491, lengthens the Arabidopsis circadian period. Affinity proteomics using a chemical probe revealed that PHA767491 binds to and inhibits multiple CKL proteins, rather than CDC7/CDK9 homologs. Simultaneous knockdown of Arabidopsis CKL-encoding genes lengthened the circadian period. CKL4 phosphorylated transcriptional repressors PSEUDO-RESPONSE REGULATOR 5 (PRR5) and TIMING OF CAB EXPRESSION 1 (TOC1) in the TTFL. PHA767491 treatment resulted in accumulation of PRR5 and TOC1, accompanied by decreasing expression of PRR5- and TOC1-target genes. A prr5 toc1 double mutant was hyposensitive to PHA767491-induced period lengthening. Together, our results reveal posttranslational modification of transcriptional repressors in plant clock TTFL by CK1 family proteins, which also modulate nonplant circadian clocks.",
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T1 - Casein kinase 1 family regulates PRR5 and TOC1 in the Arabidopsis circadian clock

AU - Uehara, Takahiro N.

AU - Mizutani, Yoshiyuki

AU - Kuwata, Keiko

AU - Hirota, Tsuyoshi

AU - Sato, Ayato

AU - Mizoi, Junya

AU - Takao, Saori

AU - Matsuo, Hiromi

AU - Suzuki, Takamasa

AU - Ito, Shogo

AU - Saito, Ami N.

AU - Nishiwaki-Ohkawa, Taeko

AU - Yamaguchi-Shinozaki, Kazuko

AU - Yoshimura, Takashi

AU - Kay, Steve A.

AU - Itami, Kenichiro

AU - Kinoshita, Toshinori

AU - Yamaguchi, Junichiro

AU - Nakamichi, Norihito

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The circadian clock provides organisms with the ability to adapt to daily and seasonal cycles. Eukaryotic clocks mostly rely on lineage-specific transcriptional-translational feedback loops (TTFLs). Posttranslational modifications are also crucial for clock functions in fungi and animals, but the posttranslational modifications that affect the plant clock are less understood. Here, using chemical biology strategies, we show that the Arabidopsis CASEIN KINASE 1 LIKE (CKL) family is involved in posttranslational modification in the plant clock. Chemical screening demonstrated that an animal CDC7/CDK9 inhibitor, PHA767491, lengthens the Arabidopsis circadian period. Affinity proteomics using a chemical probe revealed that PHA767491 binds to and inhibits multiple CKL proteins, rather than CDC7/CDK9 homologs. Simultaneous knockdown of Arabidopsis CKL-encoding genes lengthened the circadian period. CKL4 phosphorylated transcriptional repressors PSEUDO-RESPONSE REGULATOR 5 (PRR5) and TIMING OF CAB EXPRESSION 1 (TOC1) in the TTFL. PHA767491 treatment resulted in accumulation of PRR5 and TOC1, accompanied by decreasing expression of PRR5- and TOC1-target genes. A prr5 toc1 double mutant was hyposensitive to PHA767491-induced period lengthening. Together, our results reveal posttranslational modification of transcriptional repressors in plant clock TTFL by CK1 family proteins, which also modulate nonplant circadian clocks.

AB - The circadian clock provides organisms with the ability to adapt to daily and seasonal cycles. Eukaryotic clocks mostly rely on lineage-specific transcriptional-translational feedback loops (TTFLs). Posttranslational modifications are also crucial for clock functions in fungi and animals, but the posttranslational modifications that affect the plant clock are less understood. Here, using chemical biology strategies, we show that the Arabidopsis CASEIN KINASE 1 LIKE (CKL) family is involved in posttranslational modification in the plant clock. Chemical screening demonstrated that an animal CDC7/CDK9 inhibitor, PHA767491, lengthens the Arabidopsis circadian period. Affinity proteomics using a chemical probe revealed that PHA767491 binds to and inhibits multiple CKL proteins, rather than CDC7/CDK9 homologs. Simultaneous knockdown of Arabidopsis CKL-encoding genes lengthened the circadian period. CKL4 phosphorylated transcriptional repressors PSEUDO-RESPONSE REGULATOR 5 (PRR5) and TIMING OF CAB EXPRESSION 1 (TOC1) in the TTFL. PHA767491 treatment resulted in accumulation of PRR5 and TOC1, accompanied by decreasing expression of PRR5- and TOC1-target genes. A prr5 toc1 double mutant was hyposensitive to PHA767491-induced period lengthening. Together, our results reveal posttranslational modification of transcriptional repressors in plant clock TTFL by CK1 family proteins, which also modulate nonplant circadian clocks.

KW - Arabidopsis

KW - Circadian clock

KW - Posttranslational regulation

KW - Small molecule

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JF - Proceedings of the National Academy of Sciences of the United States of America

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