Cell-free mRNA translation in a microbiochemical reactor

K. Hosokawa, T. Fujii, T. Nojima, S. Shoji, A. Yotsumoto, I. Endo

研究成果: Conference contribution

3 被引用数 (Scopus)

抄録

A living cell is a huge network of chemical reactions in a compartmentalized microstructure. Realization of such a system using MEMS (microelectromechanical systems) technology would contribute to biological study and to development of an intelligent biochip. The authors have been focusing on protein synthesis in microreactors, because this process plays a central role in the chemical networks in living cells. This paper demonstrates that messenger RNA (mRNA)-polyuridylic acid-was translated into polypeptide-polyphenylalanine-in our primitive microreactor which was fabricated using conventional MEMS techniques: Silicon anisotropic etching and glass-silicon anodic bonding. The microreactor has a main reaction channel which is 14 millimeters long, 800 microns wide, and 20 microns deep. The amount of polyphenylalanine, which was synthesized in the channel, was determined using radioisotope assay.

本文言語English
ホスト出版物のタイトルMHS 1997 - Proceedings of 1997 International Symposium on Micromechatronics and Human Science
出版社Institute of Electrical and Electronics Engineers Inc.
ページ91-95
ページ数5
ISBN(電子版)0780341716, 9780780341715
DOI
出版ステータスPublished - 1997
イベント8th International Symposium on Micromechatronics and Human Science, MHS 1997 - Nagoya, Japan
継続期間: 1997 10 51997 10 8

出版物シリーズ

名前MHS 1997 - Proceedings of 1997 International Symposium on Micromechatronics and Human Science

Other

Other8th International Symposium on Micromechatronics and Human Science, MHS 1997
国/地域Japan
CityNagoya
Period97/10/597/10/8

ASJC Scopus subject areas

  • 器械工学
  • 生体医工学
  • 電子工学および電気工学
  • 機械工学
  • 電子材料、光学材料、および磁性材料

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