Cell-free translation reconstituted with purified components

Yoshihiro Shimizu; Akio Inoue; Yukihide Tomari; Tsutomu Suzuki; Takashi Yokogawa; Kazuya Nishikawa; Takuya Ueda

doi:10.1038/90802

Cell-free translation reconstituted with purified components

Yoshihiro Shimizu, Akio Inoue, Yukihide Tomari, Tsutomu Suzuki, Takashi Yokogawa, Kazuya Nishikawa, Takuya Ueda

研究成果: Article › 査読

1125 被引用数 (Scopus)

抄録

We have developed a protein-synthesizing system reconstituted from recombinant tagged protein factors purified to homogeneity. The system was able to produce protein at a rate of about 160 μg/ml/h in a batch mode without the need for any supplementary apparatus. The protein products were easily purified within 1 h using affinity chromatography to remove the tagged protein factors. Moreover, omission of a release factor allowed efficient incorporation of an unnatural amino acid using suppressor transfer RNA (tRNA).

本文言語	English
ページ（範囲）	751-755
ページ数	5
ジャーナル	Nature biotechnology
巻	19
号	8
DOI	https://doi.org/10.1038/90802
出版ステータス	Published - 2001 8 20
外部発表	はい

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Molecular Medicine
Biomedical Engineering

文献へのアクセス

10.1038/90802

フィンガープリント「Cell-free translation reconstituted with purified components」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル

@article{ee04c39a86604ed5abbbb00a3f6b94f7,

title = "Cell-free translation reconstituted with purified components",

abstract = "We have developed a protein-synthesizing system reconstituted from recombinant tagged protein factors purified to homogeneity. The system was able to produce protein at a rate of about 160 μg/ml/h in a batch mode without the need for any supplementary apparatus. The protein products were easily purified within 1 h using affinity chromatography to remove the tagged protein factors. Moreover, omission of a release factor allowed efficient incorporation of an unnatural amino acid using suppressor transfer RNA (tRNA).",

author = "Yoshihiro Shimizu and Akio Inoue and Yukihide Tomari and Tsutomu Suzuki and Takashi Yokogawa and Kazuya Nishikawa and Takuya Ueda",

year = "2001",

month = aug,

day = "20",

doi = "10.1038/90802",

language = "English",

volume = "19",

pages = "751--755",

journal = "Nature Biotechnology",

issn = "1087-0156",

publisher = "Nature Publishing Group",

number = "8",

}

TY - JOUR

T1 - Cell-free translation reconstituted with purified components

AU - Shimizu, Yoshihiro

AU - Inoue, Akio

AU - Tomari, Yukihide

AU - Suzuki, Tsutomu

AU - Yokogawa, Takashi

AU - Nishikawa, Kazuya

AU - Ueda, Takuya

PY - 2001/8/20

Y1 - 2001/8/20

N2 - We have developed a protein-synthesizing system reconstituted from recombinant tagged protein factors purified to homogeneity. The system was able to produce protein at a rate of about 160 μg/ml/h in a batch mode without the need for any supplementary apparatus. The protein products were easily purified within 1 h using affinity chromatography to remove the tagged protein factors. Moreover, omission of a release factor allowed efficient incorporation of an unnatural amino acid using suppressor transfer RNA (tRNA).

AB - We have developed a protein-synthesizing system reconstituted from recombinant tagged protein factors purified to homogeneity. The system was able to produce protein at a rate of about 160 μg/ml/h in a batch mode without the need for any supplementary apparatus. The protein products were easily purified within 1 h using affinity chromatography to remove the tagged protein factors. Moreover, omission of a release factor allowed efficient incorporation of an unnatural amino acid using suppressor transfer RNA (tRNA).

UR - http://www.scopus.com/inward/record.url?scp=0034904102&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034904102&partnerID=8YFLogxK

U2 - 10.1038/90802

DO - 10.1038/90802

M3 - Article

C2 - 11479568

AN - SCOPUS:0034904102

VL - 19

SP - 751

EP - 755

JO - Nature Biotechnology

JF - Nature Biotechnology

SN - 1087-0156

IS - 8

ER -