抄録
We have developed a protein-synthesizing system reconstituted from recombinant tagged protein factors purified to homogeneity. The system was able to produce protein at a rate of about 160 μg/ml/h in a batch mode without the need for any supplementary apparatus. The protein products were easily purified within 1 h using affinity chromatography to remove the tagged protein factors. Moreover, omission of a release factor allowed efficient incorporation of an unnatural amino acid using suppressor transfer RNA (tRNA).
本文言語 | English |
---|---|
ページ(範囲) | 751-755 |
ページ数 | 5 |
ジャーナル | Nature biotechnology |
巻 | 19 |
号 | 8 |
DOI | |
出版ステータス | Published - 2001 |
外部発表 | はい |
ASJC Scopus subject areas
- バイオテクノロジー
- バイオエンジニアリング
- 応用微生物学とバイオテクノロジー
- 分子医療
- 生体医工学