Cell-free translation system using phosphorothioate-containing mRNA.

T. Ueda*, H. Tohda, N. Chikazumi, F. Eckstein, K. Watanabe

*この研究の対応する著者

研究成果: Article査読

抄録

Phosphorothioate-containing RNAs were generated by transcription of template DNA using the Sp diastereomers of ribonucleoside 5'-O-(1-thiotriphosphates) (NTP alpha S) and T7 RNA polymerase. The substitution of mRNA by phosphorothioate increased the efficiency of protein synthesis by stabilizing the mRNAs in prokaryotic cell-free translation systems. The substituted mRNAs were also shown to be applicable to the continuous cell-free translation system developed by Spirin and coworkers.

本文言語English
ページ(範囲)151-152
ページ数2
ジャーナルNucleic acids symposium series
25
出版ステータスPublished - 1991
外部発表はい

ASJC Scopus subject areas

  • 医学(全般)

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