Cell-free translation systems for protein engineering

Yoshihiro Shimizu, Yutetsu Kuruma, Bei Wen Ying, So Umekage, Takuya Ueda

研究成果: Short survey

66 引用 (Scopus)

抜粋

Cell-free translation systems have developed significantly over the last two decades and improvements in yield have resulted in their use for protein production in the laboratory. These systems have protein engineering applications, such as the production of proteins containing unnatural amino acids and development of proteins exhibiting novel functions. Recently, it has been suggested that cell-free translation systems might be used as the fundamental basis for cell-like systems. We review recent progress in the field of cell-free translation systems and describe their use as tools for protein production and engineering.

元の言語English
ページ(範囲)4133-4140
ページ数8
ジャーナルFEBS Journal
273
発行部数18
DOI
出版物ステータスPublished - 2006 9 1
外部発表Yes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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