Cell growth control by stable Rbg2/Gir2 complex formation under amino acid starvation

Kosuke Ishikawa, Koichi Ito, Jun Ichiro Inoue, Kentaro Senba

    研究成果: Article

    2 引用 (Scopus)

    抄録

    The molecular fine-tuning mechanisms underlying adaptive responses to environmental stresses in eukaryotes remain largely unknown. Here, we report on a novel stress-induced cell growth control mechanism involving a highly conserved complex containing Rbg2 and Gir2 subunits, which are the budding yeast orthologs of human Drg2 and Dfrp2, respectively. We found that the complex is responsible for efficient cell growth under amino acid starvation. Using native PAGE analyses, we observed that, individually, Rbg2 and Gir2 were labile proteins. However, they formed a complex that stabilized each other, and this stability became significantly enhanced after amino acid starvation. We observed that the stabilization of the complex was strictly dependent on GDP or GTP binding to Rbg2. A point mutation (S77N) that inactivated nucleotide binding impaired formation of the complex and disrupted the stress-induced cell growth. Interestingly, the complex bound the translational activator Gcn1 in a dose-dependent manner according to the stress level, suggesting a dynamic association with the cellular translational machinery. We propose that the Rbg2/Gir2 complex is a modulator that maintains cellular homoeostasis, thus promoting the survival of eukaryotic organisms in stressful environments.

    元の言語English
    ページ(範囲)859-872
    ページ数14
    ジャーナルGenes to Cells
    18
    発行部数10
    DOI
    出版物ステータスPublished - 2013 10

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    Starvation
    Amino Acids
    Growth
    Native Polyacrylamide Gel Electrophoresis
    Saccharomycetales
    Guanosine Triphosphate
    Eukaryota
    Point Mutation
    Homeostasis
    Nucleotides
    Survival
    Proteins

    ASJC Scopus subject areas

    • Genetics
    • Cell Biology

    これを引用

    Cell growth control by stable Rbg2/Gir2 complex formation under amino acid starvation. / Ishikawa, Kosuke; Ito, Koichi; Inoue, Jun Ichiro; Senba, Kentaro.

    :: Genes to Cells, 巻 18, 番号 10, 10.2013, p. 859-872.

    研究成果: Article

    Ishikawa, Kosuke ; Ito, Koichi ; Inoue, Jun Ichiro ; Senba, Kentaro. / Cell growth control by stable Rbg2/Gir2 complex formation under amino acid starvation. :: Genes to Cells. 2013 ; 巻 18, 番号 10. pp. 859-872.
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