Cell-sized spherical confinement induces the spontaneous formation of contractile actomyosin rings in vitro

Makito Miyazaki, Masataka Chiba, Hiroki Eguchi, Takashi Ohki, Shin'ichi Ishiwata*

*この研究の対応する著者

    研究成果: Article査読

    82 被引用数 (Scopus)

    抄録

    During cell division, many animal cells transform into a spherical shape and assemble a contractile ring composed of actin filaments and myosin motors at the equator to separate the cell body into two. Although actomyosin regulatory proteins are spatio-temporally controlled during cytokinesis, the direct contribution of cell shape and actomyosin activity to the contractile ring assembly remains unclear. Here, we demonstrated in vitro that actin polymerization inside cell-sized spherical droplets induced the spontaneous formation of single ring-shaped actin bundles in the presence of bundling factors. Despite a lack of spatial regulatory signals, the rings always assembled at the equator to minimize the elastic energy of the bundles. Myosin promoted ring formation by the dynamic remodelling of actin networks, and an increase in the effective concentration of myosin triggered ring contraction. These results will help us understand how animal cells coordinate cell shape and actomyosin activities to direct cytokinesis.

    本文言語English
    ページ(範囲)480-489
    ページ数10
    ジャーナルNature Cell Biology
    17
    4
    DOI
    出版ステータスPublished - 2015 4月 30

    ASJC Scopus subject areas

    • 細胞生物学

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