Cellular events and behaviors after grafting of stratified squamous epithelial cell sheet onto a hydrated collagen gel

Yoshiyuki Kasai, Naoya Takeda, Shinichiro Kobayashi, Ryo Takagi, Masayuki Yamato

    研究成果: Article

    抄録

    Autologous stratified squamous epithelial cell sheets have been successfully used to treat epithelial defects in tissues such as the cornea and the esophagus. However, the regenerative cellular events occurring in the grafted epithelial cells are unclear in the early stages of wound healing. In this study, we created an in vitro grafting model using cultured normal human epidermal keratinocyte (NHEK) sheets and a type I collagen gel to investigate the cellular processes that occur within the grafted cell sheet. Cultured NHEK cells successfully became a stratified squamous cell sheet resembling epithelial tissue, retained expression of cellular integrins and adhesion proteins, and adhered successfully to a type I collagen gel. After culture on the collagen gel, expression of E-cadherin, and β-catenin decreased in the cells of the basal layer of the grafted cell sheet, resembling events characteristic of a partial epithelial-mesenchymal transition (EMT). These basal cells also induced migration of the cell sheet. Those phenomena are consistent with the essential events that occur in the wound-healing process observed previously in cell studies. Therefore, the epithelial cell sheet grafted onto a type I collagen gel is a suitable model in vitro to study cellular events and behaviors. Furthermore, we also addressed the therapeutic mechanisms by which the epithelial cell sheet promotes wound healing.

    元の言語English
    ジャーナルFEBS Open Bio
    DOI
    出版物ステータスAccepted/In press - 2017

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    Collagen
    Gels
    Epithelial Cells
    Collagen Type I
    Wound Healing
    Keratinocytes
    Cells
    Tissue
    Catenins
    Cadherins
    Epithelial-Mesenchymal Transition
    Integrins
    Adhesion
    Cornea
    Esophagus
    Cell Movement
    Epithelium
    Defects
    Proteins
    In Vitro Techniques

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

    これを引用

    Cellular events and behaviors after grafting of stratified squamous epithelial cell sheet onto a hydrated collagen gel. / Kasai, Yoshiyuki; Takeda, Naoya; Kobayashi, Shinichiro; Takagi, Ryo; Yamato, Masayuki.

    :: FEBS Open Bio, 2017.

    研究成果: Article

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    AU - Kobayashi, Shinichiro

    AU - Takagi, Ryo

    AU - Yamato, Masayuki

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    KW - Stratified squamous epithelial cell sheet

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