Changes in mobility of chromaffin granules in actin network with its assembly and Ca2+-dependent disassemby by gelsolin

S. Miyamoto, T. Funatsu, S. Ishiwata, S. Fujime*

*この研究の対応する著者

研究成果: Article査読

15 被引用数 (Scopus)

抄録

As a final stage of cell signal transduction, secretory cells release hormones by exocytosis. Before secretory granules contact with the cell membrane for fusion, an actin-network barrier must dissociate as a prelude. To elucidate dynamical behaviors of secretory granules in actin networks, in vitro assembly and disassembly processes of actin networks were examined by means of dynamic light-scattering spectroscopy. We studied actin polymerization in the presence of chromaffin granules isolated from bovine adrenal medullas and found that the entanglement of actin filaments rapidly formed cages that confined granules in them. We also studied the effect of gelsolin, one of actin-severing proteins, on the network of actin filaments preformed in the presence of chromaffin granules. It turned out that the cages that confined granules rapidly disappeared when gelsolin was added in the presence of free Ca2+ ions. A semiquantitative analysis of dynamic light-scattering spectra permitted us to estimate the changes in the mobility (or the translational diffusion coefficient) of chromaffin granules in the actin network with its assembly and Ca2+-dependent disassembly by gelsolin. Based on the present results and some pieces of evidence in the literature, a model is proposed for biophysical situations before, during, and after an exocytotic event.

本文言語English
ページ(範囲)1139-1149
ページ数11
ジャーナルBiophysical Journal
64
4
出版ステータスPublished - 1993
外部発表はい

ASJC Scopus subject areas

  • 生物理学

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