Chaotropic etching for fabricating microwells for cell culture

Nobuhiro Tamai*, Hiroyuki Moriguchi, Yuzo Takayama, Yasuhiko Jimbo, Ikuro Suzuki, Kenji Yasuda

*この研究の対応する著者

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Formation of simple neuronal networks in vitro is one of the promising methods to study biological information processing. Agarose microchambers have several advantages to form and maintain simple network structures. Here, in this work, a novel method for fabricating microwells in an agarose-layer is reported. Chaotropic effects of sodium iodide (NaI) is applied for etching agarose films. A conventional glass micropipette filled with NaI solution was aligned and a small amount of NaI was ejected to surface of the film. The agarose was denatured by the soaked NaI. The denatured agarose was washed out by distilled water. The size of the well was determined by the quantity of ejected NaI solution and its diffusion time. Conditions for fabricating wells of 100 to 600 μm diameters were established. Multiple wells up to 100 were formed on a single surface sequentially by programmed movement of the microscope stage. Rat hippocampal neurons were successfully cultured in the wells. Combining this method with microelectrode-array substrates will enable us of recording neuronal activity from simple neuronal networks as well as co-culture systems of heterogeneous tissues.

本文言語English
ページ(範囲)1568-1574+12
ジャーナルIEEJ Transactions on Electronics, Information and Systems
127
10
DOI
出版ステータスPublished - 2007
外部発表はい

ASJC Scopus subject areas

  • 電子工学および電気工学

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