Characterization and cloning of TMV resistance gene n homologues from nicotiana tabacum

Jun Shan Gao, Yan Meng, Nobumitsu Sasaki, Hiromi Kanegae, Naomi Hayashi, Hiroshi Nyunoya

研究成果: Article査読

5 被引用数 (Scopus)

抄録

Tobacco cultivars Nicotiana tabacum cv. Samsun NN plants carrying the N gene contain a multitude of N-related genes. We cloned a few N homologues and isolated two full-length cDNAs of NL-C26 and NL-B69 genes from N. tabacum cv. Samsun NN. Nucleotide sequence analysis showed that the coding regions of NL-C26 (3,498 bp) and NL-B69 (3,510 bp) had 86 and 83% nucleotide identities with the N gene, respectively. Amino acid sequence analysis revealed that NL-C26 and NL-B69 had the Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR/NBS/LRR) structure with 78 and 73% identities to N, respectively. These result indicated that NL-C26 was more similar to N than NL-B69. Tobacco mosaic virus (TMV) infection experiments suggest that NL-C26 and NL-B69 could interact with distinct avirulence (Avr) proteins of yet unidentified pathogens and function as potential HR-inducing resistance proteins similar to N.

本文言語English
ページ(範囲)7998-8006
ページ数9
ジャーナルAfrican Journal of Biotechnology
9
47
DOI
出版ステータスPublished - 2010 11 22
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 遺伝学
  • 農業および作物学

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