Characterization and tRNA recognition of mammalian mitochondrial seryl- tRNA synthetase

Takashi Yokogawa, Nobukazu Shimada, Nono Takeuchi, Lisa Benkowski, Tsutomu Suzuki, Akira Omori, Takuya Ueda, Kazuya Nishikawa, Linda L. Spremulli, Kimitsuna Watanabe*

*この研究の対応する著者

研究成果: Article査読

37 被引用数 (Scopus)

抄録

Animal mitochondrial protein synthesis systems contain two serine tRNAs (tRNAs(Ser)) corresponding to the codons AGY and UCN, each possessing an unusual secondary structure; the former lacks the entire D arm, and the latter has a slightly different cloverleaf structure. To elucidate whether these two tRNAs(Ser) can be recognized by the single animal mitochondrial seryl-tRNA synthetase (mt SerRS), we purified mt SerRS from bovine liver 2400-fold and showed that it can aminoacylate both of them. Specific interaction between mt SerRS and either of the tRNAs(Ser) was also observed in a gel retardation assay. cDNA cloning of bovine mt SerRS revealed that the deduced amino acid sequence of the enzyme contains 518 amino acid residues. The cDNAs of human and mouse mt SerRS were obtained by reverse transcription- polymerase chain reaction and expressed sequence tag data base searches. Elaborate inspection of primary sequences of mammalian mt SerRSs revealed diversity in the N-terminal domain responsible for tRNA recognition, indicating that the recognition mechanism of mammalian mt SerRS differs considerably from that of its prokaryotic counterpart. In addition, the human mt SerRS gene was found to be located on chromosome 19q13.1, to which the autosomal deafness locus DFNA4 is mapped.

本文言語English
ページ(範囲)19913-19920
ページ数8
ジャーナルJournal of Biological Chemistry
275
26
DOI
出版ステータスPublished - 2000 6月 30
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Characterization and tRNA recognition of mammalian mitochondrial seryl- tRNA synthetase」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル