Characterization of a putative promoter region of the human poly(ADP-ribose) polymerase gene: Structural similarity to that of the DNA polymerase β gene

Tsutomu Ogura*, Hiroshi Nyunoya, Mitsuko Takahashi-Masutani, Masanao Miwa, Takashi Sugimura, Hiroyasu Esumi

*この研究の対応する著者

研究成果: Article査読

36 被引用数 (Scopus)

抄録

The 5′-flanking region of the human poly(ADP-ribose) polymerase gene was isolated and characterized. The nucleotide sequence of a part of the poly(ADP-ribose) polymerase gene completely matched that of the cDNA. The transcriptional initiation sites (cap sites) of this gene, located about 166-bp upstream from the translational initiation site, were identified by S1 mapping analysis. Neither CAAT box nor TATA box was found within 500-bp upstream from the cap sites of poly(ADP-ribose) polymerase gene. The 200-bp immediately upstream of the cap site had a high G+C content (76.5%) and contained double repeats of the sequence CCGCCC, putative Sp1 binding sites, and a palindromic structure. The 5′-flanking region of poly(ADP-ribose) polymerase gene also showed promoter activity in chloramphenicol acetyltransferase assay and structural similarity to that of DNA polymerase β gene.

本文言語English
ページ(範囲)701-710
ページ数10
ジャーナルBiochemical and Biophysical Research Communications
167
2
DOI
出版ステータスPublished - 1990 3 16
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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