Characterization of denitrifying polyphosphate-accumulating organisms in activated sludge based on nitrite reductase gene

Satoshi Tsuneda*, Ryuki Miyauchi, Takashi Ohno, Akira Hirata

*この研究の対応する著者

研究成果: Article査読

53 被引用数 (Scopus)

抄録

Nitrite reductase gene (nirS) fragments in the activated sludge obtained from a sequencing batch reactor (SBR) under anaerobic-aerobic condition were cloned and classified by restriction fragment length polymorphism (RFLP) analysis, and representative fragments were sequenced. One of the nirS clones was approximately 70% of all nirS clones in anaerobic/aerobic (existing oxygen and nitrate) cycle operation in which a large amount of anoxic phosphate uptake was observed. Although the activated sludge samples analyzed might contain bacteria that did not accumulate polyphosphate, it was likely that this nirS fragment sequence was that from denitrifying polyphosphate-accumulating organisms (DNPAOs) which can utilize both oxygen and nitrate as electron acceptors. The sequence was similar to the nirS sequences of Thauera mechernichensis (83% similarity) and Azoarcus tolulyticus (83% similarity) both of which belong to the Rhodocyclus group.

本文言語English
ページ(範囲)403-407
ページ数5
ジャーナルJournal of Bioscience and Bioengineering
99
4
DOI
出版ステータスPublished - 2005 4月

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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