Characterization of denitrifying polyphosphate-accumulating organisms in activated sludge based on nitrite reductase gene

Satoshi Tsuneda, Ryuki Miyauchi, Takashi Ohno, Akira Hirata

研究成果: Article

44 引用 (Scopus)

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Nitrite reductase gene (nirS) fragments in the activated sludge obtained from a sequencing batch reactor (SBR) under anaerobic-aerobic condition were cloned and classified by restriction fragment length polymorphism (RFLP) analysis, and representative fragments were sequenced. One of the nirS clones was approximately 70% of all nirS clones in anaerobic/aerobic (existing oxygen and nitrate) cycle operation in which a large amount of anoxic phosphate uptake was observed. Although the activated sludge samples analyzed might contain bacteria that did not accumulate polyphosphate, it was likely that this nirS fragment sequence was that from denitrifying polyphosphate-accumulating organisms (DNPAOs) which can utilize both oxygen and nitrate as electron acceptors. The sequence was similar to the nirS sequences of Thauera mechernichensis (83% similarity) and Azoarcus tolulyticus (83% similarity) both of which belong to the Rhodocyclus group.

元の言語English
ページ(範囲)403-407
ページ数5
ジャーナルJournal of Bioscience and Bioengineering
99
発行部数4
DOI
出版物ステータスPublished - 2005 4

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ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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