Characterization of denitrifying polyphosphate-accumulating organisms in activated sludge based on nitrite reductase gene

Satoshi Tsuneda, Ryuki Miyauchi, Takashi Ohno, Akira Hirata

    研究成果: Article

    43 引用 (Scopus)

    抄録

    Nitrite reductase gene (nirS) fragments in the activated sludge obtained from a sequencing batch reactor (SBR) under anaerobic-aerobic condition were cloned and classified by restriction fragment length polymorphism (RFLP) analysis, and representative fragments were sequenced. One of the nirS clones was approximately 70% of all nirS clones in anaerobic/aerobic (existing oxygen and nitrate) cycle operation in which a large amount of anoxic phosphate uptake was observed. Although the activated sludge samples analyzed might contain bacteria that did not accumulate polyphosphate, it was likely that this nirS fragment sequence was that from denitrifying polyphosphate-accumulating organisms (DNPAOs) which can utilize both oxygen and nitrate as electron acceptors. The sequence was similar to the nirS sequences of Thauera mechernichensis (83% similarity) and Azoarcus tolulyticus (83% similarity) both of which belong to the Rhodocyclus group.

    元の言語English
    ページ(範囲)403-407
    ページ数5
    ジャーナルJournal of Bioscience and Bioengineering
    99
    発行部数4
    DOI
    出版物ステータスPublished - 2005 4

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    Nitrite Reductases
    Polyphosphates
    Sewage
    Nitrates
    Rhodocyclaceae
    Thauera
    Azoarcus
    Clone Cells
    Genes
    Oxygen
    Batch reactors
    Polymorphism
    Restriction Fragment Length Polymorphisms
    Bacteria
    Phosphates
    Electrons
    Oxidoreductases

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering

    これを引用

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    abstract = "Nitrite reductase gene (nirS) fragments in the activated sludge obtained from a sequencing batch reactor (SBR) under anaerobic-aerobic condition were cloned and classified by restriction fragment length polymorphism (RFLP) analysis, and representative fragments were sequenced. One of the nirS clones was approximately 70{\%} of all nirS clones in anaerobic/aerobic (existing oxygen and nitrate) cycle operation in which a large amount of anoxic phosphate uptake was observed. Although the activated sludge samples analyzed might contain bacteria that did not accumulate polyphosphate, it was likely that this nirS fragment sequence was that from denitrifying polyphosphate-accumulating organisms (DNPAOs) which can utilize both oxygen and nitrate as electron acceptors. The sequence was similar to the nirS sequences of Thauera mechernichensis (83{\%} similarity) and Azoarcus tolulyticus (83{\%} similarity) both of which belong to the Rhodocyclus group.",
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    AU - Miyauchi, Ryuki

    AU - Ohno, Takashi

    AU - Hirata, Akira

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    N2 - Nitrite reductase gene (nirS) fragments in the activated sludge obtained from a sequencing batch reactor (SBR) under anaerobic-aerobic condition were cloned and classified by restriction fragment length polymorphism (RFLP) analysis, and representative fragments were sequenced. One of the nirS clones was approximately 70% of all nirS clones in anaerobic/aerobic (existing oxygen and nitrate) cycle operation in which a large amount of anoxic phosphate uptake was observed. Although the activated sludge samples analyzed might contain bacteria that did not accumulate polyphosphate, it was likely that this nirS fragment sequence was that from denitrifying polyphosphate-accumulating organisms (DNPAOs) which can utilize both oxygen and nitrate as electron acceptors. The sequence was similar to the nirS sequences of Thauera mechernichensis (83% similarity) and Azoarcus tolulyticus (83% similarity) both of which belong to the Rhodocyclus group.

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    KW - Activated sludge

    KW - Denitrifying polyphosphate-accumulating organisms (DNPAOs)

    KW - Enhanced biological phosphorus removal (EBPR)

    KW - Nitrite reductase gene (nirS)

    KW - Polymerase chain reaction (PCR)

    KW - Polyphosphate-accumulating organisms (PAOs)

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