Characterization of direct immobilized probe DNA on partially functionalized diamond solution-gate field-effect transistors

Jung Hoon Yang, Kwang Soup Song, Shouma Kuga, Hiroshi Kawarada*

*この研究の対応する著者

研究成果: Article査読

19 被引用数 (Scopus)

抄録

Amino groups were functionalized directly on the diamond surface after treating 0.5 monolayer of oxidation for detection of DNAs. Also, immobilization of probe DNAs was carried out directly on the partially aminated diamond without linker molecules. Specific hybridization with 21-me.r DNA at a concentration of 10OnM could be clearly detected by two methods, fluorescence microscopy and diamond solution-gate field-effect transistors (SGFETs). DNA hybridization was confirmed using Cy-5-labeled target DNA on a micropatterned diamond surface. The changes in gate potential by the negative charge of immobilized or hybridized DNA were measured on SGFETs and hybridization efficiency on the functionalized diamond surface was estimated as about 40%.

本文言語English
ページ(範囲)L1114-L1117
ジャーナルJapanese Journal of Applied Physics, Part 2: Letters
45
42-45
DOI
出版ステータスPublished - 2006 11月 10

ASJC Scopus subject areas

  • 工学(全般)
  • 物理学および天文学(その他)
  • 物理学および天文学(全般)

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