Characterization of DNA hybridization on partially aminated diamond by aromatic compounds

Jung Hoon Yang, Kwang Soup Song, Guo Jun Zhang, Munenori Degawa, Yoshinori Sasaki, Iwao Ohdomari, Hiroshi Kawarada

研究成果: Article査読

41 被引用数 (Scopus)

抄録

Here, we report a novel method of micropatterning oligonucleotides via aromatic groups as linkers on partially amino-terminated diamond and the inherence on subsequent hybridization. The covalent immobilization of probe oligonucleotides and characterization of immobilized probe oligonucleotides with carboxylic compounds were investigated by X-ray photoelectron spectroscopy (XPS). To confirm the effects of linker flexibility in a low amino group on diamond for probe oligonucleotides, three kinds of dicarboxylic compound - adipic acid, terephthalic acid, and trimesic acid - were used for immobilization of probe oligonucleotides, like linkers; and these oligonucleotides were hybridized with target oligonucleotides labeled with Cy 5 on the micropatterned diamond surface. The hybridization intensities determined by epifluorescence microscopy were compared and analyzed.

本文言語English
ページ(範囲)11245-11250
ページ数6
ジャーナルLangmuir
22
26
DOI
出版ステータスPublished - 2006 12 19

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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