Characterization of iron uptake in the magnetic bacterium Aquaspirillum sp. AMB-1

Chikashi Nakamura, Toshifumi Sakaguchi, Satoko Kudo, J. Grant Burgess, Koji Sode, Tadashi Matsunaga

研究成果: Article

16 引用 (Scopus)

抜粋

The aim of this work is to elucidate the iron uptake mechanism in the magnetic bacterium Aquaspirillum sp. AMB-1, which can utilize a wide variety of iron chelates. However, no siderophore could be detected in spent culture. A 400-bp DNA fragment was amplified by polymerase chain reaction (PCR) from the genomic DNA of AMB-1, using primers designed from the sfuC gene of Serratia marcescens. This gene encodes a nucleotide binding protein in the periplasmic binding protein (PBP) dependent iron transport system. The amplified fragment was homologous with the conserved sequence of sfuC. Our results suggest that a nucleotide binding protein mediated iron transport system similar to that observed in Serratia marcescens is present in Aquaspirillum sp. AMB-1.

元の言語English
ページ(範囲)169-176
ページ数8
ジャーナルApplied Biochemistry and Biotechnology
39-40
発行部数1
DOI
出版物ステータスPublished - 1993 9
外部発表Yes

    フィンガープリント

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

Nakamura, C., Sakaguchi, T., Kudo, S., Burgess, J. G., Sode, K., & Matsunaga, T. (1993). Characterization of iron uptake in the magnetic bacterium Aquaspirillum sp. AMB-1. Applied Biochemistry and Biotechnology, 39-40(1), 169-176. https://doi.org/10.1007/BF02918987