Characterization of iron uptake in the magnetic bacterium Aquaspirillum sp. AMB-1

Chikashi Nakamura, Toshifumi Sakaguchi, Satoko Kudo, J. Grant Burgess, Koji Sode, Tadashi Matsunaga*

*この研究の対応する著者

研究成果: Article査読

16 被引用数 (Scopus)

抄録

The aim of this work is to elucidate the iron uptake mechanism in the magnetic bacterium Aquaspirillum sp. AMB-1, which can utilize a wide variety of iron chelates. However, no siderophore could be detected in spent culture. A 400-bp DNA fragment was amplified by polymerase chain reaction (PCR) from the genomic DNA of AMB-1, using primers designed from the sfuC gene of Serratia marcescens. This gene encodes a nucleotide binding protein in the periplasmic binding protein (PBP) dependent iron transport system. The amplified fragment was homologous with the conserved sequence of sfuC. Our results suggest that a nucleotide binding protein mediated iron transport system similar to that observed in Serratia marcescens is present in Aquaspirillum sp. AMB-1.

本文言語English
ページ(範囲)169-176
ページ数8
ジャーナルApplied Biochemistry and Biotechnology
39-40
1
DOI
出版ステータスPublished - 1993 9
外部発表はい

ASJC Scopus subject areas

  • バイオエンジニアリング
  • バイオテクノロジー
  • 生化学
  • 生化学、遺伝学、分子生物学(全般)

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