抄録
The aim of this work is to elucidate the iron uptake mechanism in the magnetic bacterium Aquaspirillum sp. AMB-1, which can utilize a wide variety of iron chelates. However, no siderophore could be detected in spent culture. A 400-bp DNA fragment was amplified by polymerase chain reaction (PCR) from the genomic DNA of AMB-1, using primers designed from the sfuC gene of Serratia marcescens. This gene encodes a nucleotide binding protein in the periplasmic binding protein (PBP) dependent iron transport system. The amplified fragment was homologous with the conserved sequence of sfuC. Our results suggest that a nucleotide binding protein mediated iron transport system similar to that observed in Serratia marcescens is present in Aquaspirillum sp. AMB-1.
| 本文言語 | English |
|---|---|
| ページ（範囲） | 169-176 |
| ページ数 | 8 |
| ジャーナル | Applied Biochemistry and Biotechnology |
| 巻 | 39-40 |
| 号 | 1 |
| DOI | |
| 出版ステータス | Published - 1993 9 |
| 外部発表 | はい |
ASJC Scopus subject areas
- バイオエンジニアリング
- バイオテクノロジー
- 生化学
- 生化学、遺伝学、分子生物学（全般）