Characterization of rat placental lactogen-α (PL-α) with an antipeptide antibody directed against rat PLs

N. Hattori, M. Wakimasu, M. Takahashi, M. Hirosawa, K. Shiota, T. Ogawa

研究成果: Article

10 引用 (Scopus)

抜粋

We have already reported that the serum of the mid-pregnant rat contains placental lactogen-α (PL-α), which has a higher molecular weight than PL-I when analysed by gel-filtration chromatography. In order to characterize PL- α, we prepared antipeptide antisera directed against the hydrophilic regions of authentic PLs and developed RIAs with them. With these RIAs, antiserum AI- 86 reacted only with PL-I, whereas antiserum AII-86 reacted with both PL-α and PL-I, which indicates that the antibody recognition sites of these molecules are not identical and their core proteins differ distinctly. As the use of the RIAs with AI-86 and AII-86 in combination enabled PL-α and PL-I to be discriminated, the glycoresidues and hydrophobicity of PL-α were analyzed further. Analysis with hydrophobic chromatography revealed that PL- α was less hydrophobic than PL-I and, furthermore, PL-α, but not PL-I, bound specifically to a wheat germ agglutinin (WGA) lectin column. Therefore, in view of their different amino acid sequences and glycoresidues, PL-α and PL-I are distinct molecular entities.

元の言語English
ページ(範囲)727-735
ページ数9
ジャーナルEndocrine Journal
40
発行部数6
出版物ステータスPublished - 1993
外部発表Yes

ASJC Scopus subject areas

  • Endocrinology

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  • これを引用

    Hattori, N., Wakimasu, M., Takahashi, M., Hirosawa, M., Shiota, K., & Ogawa, T. (1993). Characterization of rat placental lactogen-α (PL-α) with an antipeptide antibody directed against rat PLs. Endocrine Journal, 40(6), 727-735.