Characterization of rat placental lactogen-α (PL-α) with an antipeptide antibody directed against rat PLs

N. Hattori, M. Wakimasu, M. Takahashi, M. Hirosawa, K. Shiota, T. Ogawa

研究成果: Article

10 引用 (Scopus)

抄録

We have already reported that the serum of the mid-pregnant rat contains placental lactogen-α (PL-α), which has a higher molecular weight than PL-I when analysed by gel-filtration chromatography. In order to characterize PL- α, we prepared antipeptide antisera directed against the hydrophilic regions of authentic PLs and developed RIAs with them. With these RIAs, antiserum AI- 86 reacted only with PL-I, whereas antiserum AII-86 reacted with both PL-α and PL-I, which indicates that the antibody recognition sites of these molecules are not identical and their core proteins differ distinctly. As the use of the RIAs with AI-86 and AII-86 in combination enabled PL-α and PL-I to be discriminated, the glycoresidues and hydrophobicity of PL-α were analyzed further. Analysis with hydrophobic chromatography revealed that PL- α was less hydrophobic than PL-I and, furthermore, PL-α, but not PL-I, bound specifically to a wheat germ agglutinin (WGA) lectin column. Therefore, in view of their different amino acid sequences and glycoresidues, PL-α and PL-I are distinct molecular entities.

元の言語English
ページ(範囲)727-735
ページ数9
ジャーナルEndocrine Journal
40
発行部数6
出版物ステータスPublished - 1993
外部発表Yes

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Placental Lactogen
Antibodies
Immune Sera
Wheat Germ Agglutinins
rat placental lactogen I
Hydrophobic and Hydrophilic Interactions
Gel Chromatography
Chromatography
Amino Acid Sequence
Molecular Weight

ASJC Scopus subject areas

  • Endocrinology

これを引用

Hattori, N., Wakimasu, M., Takahashi, M., Hirosawa, M., Shiota, K., & Ogawa, T. (1993). Characterization of rat placental lactogen-α (PL-α) with an antipeptide antibody directed against rat PLs. Endocrine Journal, 40(6), 727-735.

Characterization of rat placental lactogen-α (PL-α) with an antipeptide antibody directed against rat PLs. / Hattori, N.; Wakimasu, M.; Takahashi, M.; Hirosawa, M.; Shiota, K.; Ogawa, T.

:: Endocrine Journal, 巻 40, 番号 6, 1993, p. 727-735.

研究成果: Article

Hattori, N, Wakimasu, M, Takahashi, M, Hirosawa, M, Shiota, K & Ogawa, T 1993, 'Characterization of rat placental lactogen-α (PL-α) with an antipeptide antibody directed against rat PLs', Endocrine Journal, 巻. 40, 番号 6, pp. 727-735.
Hattori N, Wakimasu M, Takahashi M, Hirosawa M, Shiota K, Ogawa T. Characterization of rat placental lactogen-α (PL-α) with an antipeptide antibody directed against rat PLs. Endocrine Journal. 1993;40(6):727-735.
Hattori, N. ; Wakimasu, M. ; Takahashi, M. ; Hirosawa, M. ; Shiota, K. ; Ogawa, T. / Characterization of rat placental lactogen-α (PL-α) with an antipeptide antibody directed against rat PLs. :: Endocrine Journal. 1993 ; 巻 40, 番号 6. pp. 727-735.
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