Characterization of the promoter region of the c-yes proto-oncogene: The importance of the GC boxes on its promoter activity

Y. Matsuzawa, K. Semba, J. Kawamura-Tsuzukul, T. Sudo, S. Ishii, K. Toyoshima, T. Yamamoto

研究成果: Article

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In this study we cloned the 5' flanking sequence of the human c-yes gene and identified its promoter region. A 0.53 kilobase pair (kbp) fragment containing the 5' terminus of the c-yes gene showed strong promoter activity when placed upstream of the bacterial chloramphenicol acetyltransferase (CAT) gene and transfected into monkey CV-1 cells. By nuclease S1 mapping multiple transcriptional start sites were detected within the promoter region. Nucleotide sequence analysis revealed that the c-yes promoter region had high G + C contents (64%) and contained six GC box-like sequences (one at the 5' distal region and five in a cluster at the 5' proximal region), but not a TATA box. These features of the c-yes promoter region are similar to those of other proto-oncogenes, ras-family genes and c-raf-1, and some housekeeping genes. Deletion analysis suggested that the most downstream 0.21 kbp region is primarily important for the promoter activity. This 0.21 kbp region contains one major and another minor transcriptional start site. Five GC box-like sequences were located within this region, and four of them were shown to bind with purified Sp1 transcription factor. Furthermore, using the base-substituted mutants of the Sp1-binding sites, each GC box in the cluster (GCl to GC4) was shown to affect the c-yes gene expression.

元の言語English
ページ(範囲)1561-1567
ページ数7
ジャーナルOncogene
6
発行部数9
出版物ステータスPublished - 1991 10 23
外部発表Yes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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    Matsuzawa, Y., Semba, K., Kawamura-Tsuzukul, J., Sudo, T., Ishii, S., Toyoshima, K., & Yamamoto, T. (1991). Characterization of the promoter region of the c-yes proto-oncogene: The importance of the GC boxes on its promoter activity. Oncogene, 6(9), 1561-1567.