Characterization of the promoter region of the src family gene lyn and its trans activation by human T-cell leukemia virus type I-encoded p40(tax)

F. Uchiumi, K. Semba, Y. Yamanashi, J. I. Fujisawa, M. Yoshida, K. Inoue, K. Toyoshima, T. Yamamoto*

*この研究の対応する著者

研究成果査読

41 被引用数 (Scopus)

抄録

The src family gene lyn is expressed preferentially in B lymphocytes but very little in normal T lymphocytes. Transcription of the lyn gene in T lymphocytes was shown to be induced by the p40(tax) protein encoded by human T-cell lymphotropic virus type I. For determination of the mechanism of p40(tax)-mediated trans activation, the transcriptional promoter region of the lyn gene was characterized. By endonuclease S1 mapping, the transcriptional initiation sites were identified within the 770-bp EcoRI-SacI fragment of the 5'-terminal portion of the human lyn gene. This fragment showed promoter activity when placed upstream of the bacterial chloramphenicol acetyltransferase gene and transfected into various cell lines. Nucleotide sequence analysis revealed that the lyn promoter region contained four GC box-like sequences but not a TATA or CCAAT box. In addition, it contained sequences characteristic of a cyclic AMP-responsive element, octamer-binding motif, PEA3-like motifs, and NFκB-binding motif- like sequence. Mutational analysis suggested that the octamer-binding motif sequence is of primary importance for the lyn promoter activity but that the other elements are not. Cotransfection of various chloramphenicol acetyltransferase constructs containing different length of the lyn promoter together with p40(tax) expression plasmids into Jurkat T cells showed that the sequence responsible for p40(tax)-induced transcription is present around the transcription initiation sites.

本文言語English
ページ(範囲)3784-3795
ページ数12
ジャーナルMolecular and Cellular Biology
12
9
DOI
出版ステータスPublished - 1992
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 細胞生物学

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