Characterizing and modulating the mechanical properties of hydrogels from ventricular extracellular matrix

Kyohei Fujita, Yuuki Tuchida, Hiroki Seki, Tadashi Kosawada, Zhonggang Feng, Yasuyuki Shiraishi, Daisuke Sato, Takao Nakamura, Mitsuo Umezu

研究成果: Conference contribution

4 被引用数 (Scopus)

抄録

In order to differentiate pluripotent stem cells to cardiomyocytes, the most general method is to expose stem cells to various growth factors related to cardiogenesis. However, a novel method has been reported to induce cardiac differentiation of human ES cells without supplemental growth factors by culturing embryoid body of human ES cells in hybrid gels composed of cardiac extracellular matrix (ECM) and type I collagen. On the other hand, mechanical properties of scaffold is one of the critical cue for differentiation of stem cells. However, it has not been thoroughly investigated the mechanical properties of the scaffold made from cardiac ECM in view of this and other reports about the differentiation of stem cells into cardiomyocytes using cardiac ECM scaffold. In this study, we fabricated bio-hydrogels composed of goat ventricular extracellular matrix, and investigated the mechanical properties by means of uniaxial compression test. It showed that the ECM gels possess viscoelastic property. The elastic modulus K1 in modified non-linear Kelvin model is 9.5 Pa for these gels and K2 is 814.7 Pa. Moreover, we were able to improve the elastic moduli K1 and K2 up to 139.7 Pa and 2023.9 Pa, respectively, by chemical treatment using EDAC.

本文言語English
ホスト出版物のタイトル2015 10th Asian Control Conference
ホスト出版物のサブタイトルEmerging Control Techniques for a Sustainable World, ASCC 2015
編集者Hazlina Selamat, Hafiz Rashidi Haruna Ramli, Ahmad Athif Mohd Faudzi, Ribhan Zafira Abdul Rahman, Asnor Juraiza Ishak, Azura Che Soh, Siti Anom Ahmad
出版社Institute of Electrical and Electronics Engineers Inc.
ISBN(電子版)9781479978625
DOI
出版ステータスPublished - 2015 9 8
イベント10th Asian Control Conference, ASCC 2015 - Kota Kinabalu, Malaysia
継続期間: 2015 5 312015 6 3

出版物シリーズ

名前2015 10th Asian Control Conference: Emerging Control Techniques for a Sustainable World, ASCC 2015

Other

Other10th Asian Control Conference, ASCC 2015
CountryMalaysia
CityKota Kinabalu
Period15/5/3115/6/3

ASJC Scopus subject areas

  • Control and Systems Engineering

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