TY - GEN
T1 - Characterizing and modulating the mechanical properties of hydrogels from ventricular extracellular matrix
AU - Fujita, Kyohei
AU - Tuchida, Yuuki
AU - Seki, Hiroki
AU - Kosawada, Tadashi
AU - Feng, Zhonggang
AU - Shiraishi, Yasuyuki
AU - Sato, Daisuke
AU - Nakamura, Takao
AU - Umezu, Mitsuo
N1 - Publisher Copyright:
© 2015 IEEE.
PY - 2015/9/8
Y1 - 2015/9/8
N2 - In order to differentiate pluripotent stem cells to cardiomyocytes, the most general method is to expose stem cells to various growth factors related to cardiogenesis. However, a novel method has been reported to induce cardiac differentiation of human ES cells without supplemental growth factors by culturing embryoid body of human ES cells in hybrid gels composed of cardiac extracellular matrix (ECM) and type I collagen. On the other hand, mechanical properties of scaffold is one of the critical cue for differentiation of stem cells. However, it has not been thoroughly investigated the mechanical properties of the scaffold made from cardiac ECM in view of this and other reports about the differentiation of stem cells into cardiomyocytes using cardiac ECM scaffold. In this study, we fabricated bio-hydrogels composed of goat ventricular extracellular matrix, and investigated the mechanical properties by means of uniaxial compression test. It showed that the ECM gels possess viscoelastic property. The elastic modulus K1 in modified non-linear Kelvin model is 9.5 Pa for these gels and K2 is 814.7 Pa. Moreover, we were able to improve the elastic moduli K1 and K2 up to 139.7 Pa and 2023.9 Pa, respectively, by chemical treatment using EDAC.
AB - In order to differentiate pluripotent stem cells to cardiomyocytes, the most general method is to expose stem cells to various growth factors related to cardiogenesis. However, a novel method has been reported to induce cardiac differentiation of human ES cells without supplemental growth factors by culturing embryoid body of human ES cells in hybrid gels composed of cardiac extracellular matrix (ECM) and type I collagen. On the other hand, mechanical properties of scaffold is one of the critical cue for differentiation of stem cells. However, it has not been thoroughly investigated the mechanical properties of the scaffold made from cardiac ECM in view of this and other reports about the differentiation of stem cells into cardiomyocytes using cardiac ECM scaffold. In this study, we fabricated bio-hydrogels composed of goat ventricular extracellular matrix, and investigated the mechanical properties by means of uniaxial compression test. It showed that the ECM gels possess viscoelastic property. The elastic modulus K1 in modified non-linear Kelvin model is 9.5 Pa for these gels and K2 is 814.7 Pa. Moreover, we were able to improve the elastic moduli K1 and K2 up to 139.7 Pa and 2023.9 Pa, respectively, by chemical treatment using EDAC.
KW - cardiac extracellular matrix
KW - hydrogel
KW - viscoelasticity
UR - http://www.scopus.com/inward/record.url?scp=84957717746&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84957717746&partnerID=8YFLogxK
U2 - 10.1109/ASCC.2015.7244661
DO - 10.1109/ASCC.2015.7244661
M3 - Conference contribution
AN - SCOPUS:84957717746
T3 - 2015 10th Asian Control Conference: Emerging Control Techniques for a Sustainable World, ASCC 2015
BT - 2015 10th Asian Control Conference
A2 - Selamat, Hazlina
A2 - Ramli, Hafiz Rashidi Haruna
A2 - Faudzi, Ahmad Athif Mohd
A2 - Rahman, Ribhan Zafira Abdul
A2 - Ishak, Asnor Juraiza
A2 - Soh, Azura Che
A2 - Ahmad, Siti Anom
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 10th Asian Control Conference, ASCC 2015
Y2 - 31 May 2015 through 3 June 2015
ER -