Chd2 interacts with H3.3 to determine myogenic cell fate

Akihito Harada, Seiji Okada, Daijiro Konno, Jun Odawara, Tomohiko Yoshimi, Saori Yoshimura, Hiromi Kumamaru, Hirokazu Saiwai, Toshiaki Tsubota, Hitoshi Kurumizaka, Koichi Akashi, Taro Tachibana, Anthony N. Imbalzano, Yasuyuki Ohkawa*

*この研究の対応する著者

    研究成果: Article査読

    87 被引用数 (Scopus)

    抄録

    Cell differentiation is mediated by lineage-determining transcription factors. We show that chromodomain helicase DNA-binding domain 2 (Chd2), a SNF2 chromatin remodelling enzyme family member, interacts with MyoD and myogenic gene regulatory sequences to specifically mark these loci via deposition of the histone variant H3.3 prior to cell differentiation. Directed and genome-wide analysis of endogenous H3.3 incorporation demonstrates that knockdown of Chd2 prevents H3.3 deposition at differentiation-dependent, but not housekeeping, genes and inhibits myogenic gene activation. The data indicate that MyoD determines cell fate and facilitates differentiation-dependent gene expression through Chd2-dependent deposition of H3.3 at myogenic loci prior to differentiation.

    本文言語English
    ページ(範囲)2994-3007
    ページ数14
    ジャーナルEMBO Journal
    31
    13
    DOI
    出版ステータスPublished - 2012 7月 4

    ASJC Scopus subject areas

    • 分子生物学
    • 生化学、遺伝学、分子生物学(全般)
    • 免疫学および微生物学(全般)
    • 神経科学(全般)

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