Chemiluminescence Enzyms lmmunoassay Using Bacterial Magnetic Particles

Tadashi Matsunaga, Masashi Kawasaki, Xie Yu, Noriyuki Tsujimura, Noriyuki Nakamura

研究成果: Article

108 引用 (Scopus)

抄録

A novel chemiluminescence enzyme immunoassay using bacterial magnetic particles (BMPs) has been developed for highly sensitive and rapid detection of immunoglobulin G. Antibody was immobilized onto BMPs using the heterobifunctional reagents sulfosuccinimidyl 6-[3'-(2-pyridyldithio)propionamido]hexanoate (sulfo-LC-SPDP) and sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1 -carboxylate (sulfo-SMCC). For the highly sensitive immunoassay method using these BMPs, a good relationship was obtained between the luminescence intensity and mouse IgG concentration in the range of 1-105 fg/mL. Furthermore, in order to reduce assay time and to simplify operations, a rapid chemiluminescence enzyme immunoassay method has been developed. The rapid method was completed in 10 min. A linear relationship was obtained between the luminescence and mouse IgG concentration hi the range of 10-1000 ng/mL,.

元の言語English
ページ(範囲)3551-3554
ページ数4
ジャーナルAnalytical Chemistry
68
発行部数20
出版物ステータスPublished - 1996 10 15
外部発表Yes

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Chemiluminescence
Immunoglobulin G
Luminescence
Enzymes
Assays
Antibodies
sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate

ASJC Scopus subject areas

  • Analytical Chemistry

これを引用

Matsunaga, T., Kawasaki, M., Yu, X., Tsujimura, N., & Nakamura, N. (1996). Chemiluminescence Enzyms lmmunoassay Using Bacterial Magnetic Particles. Analytical Chemistry, 68(20), 3551-3554.

Chemiluminescence Enzyms lmmunoassay Using Bacterial Magnetic Particles. / Matsunaga, Tadashi; Kawasaki, Masashi; Yu, Xie; Tsujimura, Noriyuki; Nakamura, Noriyuki.

:: Analytical Chemistry, 巻 68, 番号 20, 15.10.1996, p. 3551-3554.

研究成果: Article

Matsunaga, T, Kawasaki, M, Yu, X, Tsujimura, N & Nakamura, N 1996, 'Chemiluminescence Enzyms lmmunoassay Using Bacterial Magnetic Particles', Analytical Chemistry, 巻. 68, 番号 20, pp. 3551-3554.
Matsunaga T, Kawasaki M, Yu X, Tsujimura N, Nakamura N. Chemiluminescence Enzyms lmmunoassay Using Bacterial Magnetic Particles. Analytical Chemistry. 1996 10 15;68(20):3551-3554.
Matsunaga, Tadashi ; Kawasaki, Masashi ; Yu, Xie ; Tsujimura, Noriyuki ; Nakamura, Noriyuki. / Chemiluminescence Enzyms lmmunoassay Using Bacterial Magnetic Particles. :: Analytical Chemistry. 1996 ; 巻 68, 番号 20. pp. 3551-3554.
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