Chemiluminescence Enzyms lmmunoassay Using Bacterial Magnetic Particles

Tadashi Matsunaga*, Masashi Kawasaki, Xie Yu, Noriyuki Tsujimura, Noriyuki Nakamura

*この研究の対応する著者

研究成果査読

110 被引用数 (Scopus)

抄録

A novel chemiluminescence enzyme immunoassay using bacterial magnetic particles (BMPs) has been developed for highly sensitive and rapid detection of immunoglobulin G. Antibody was immobilized onto BMPs using the heterobifunctional reagents sulfosuccinimidyl 6-[3'-(2-pyridyldithio)propionamido]hexanoate (sulfo-LC-SPDP) and sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1 -carboxylate (sulfo-SMCC). For the highly sensitive immunoassay method using these BMPs, a good relationship was obtained between the luminescence intensity and mouse IgG concentration in the range of 1-105 fg/mL. Furthermore, in order to reduce assay time and to simplify operations, a rapid chemiluminescence enzyme immunoassay method has been developed. The rapid method was completed in 10 min. A linear relationship was obtained between the luminescence and mouse IgG concentration hi the range of 10-1000 ng/mL,.

本文言語English
ページ(範囲)3551-3554
ページ数4
ジャーナルAnalytical Chemistry
68
20
出版ステータスPublished - 1996 10 15
外部発表はい

ASJC Scopus subject areas

  • 分析化学

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