Chitosanase displayed on liposome can increase its activity and stability

Kien Xuan Ngo, Hiroshi Umakoshi, Toshinori Shimanouchi, Hiroyuki Sugaya, Ryoichi Kuboi

研究成果: Article

抜粋

The strategy to prepare a novel biocatalyst by the immobilization of chitosanase onto liposome (ICL) was carried out based on the direct interaction of liposomes with cell membrane of Streptomyces griseus cell. The ICL was characterized in relation to the molecular weight of protein, the chitosanase activity, the effect of the surface hydration of various liposomes on hydrolysis activity of immobilized chitosanase and the stability of ICL under various extreme conditions. The SDS-PAGE analysis of the purified ICL sample shows the existence of a protein with approximately 39 kDa that corresponded to the sum of weight of the mature chitosanase and its signal peptide (38.8 kDa). The above protein of ICL also expresses the chitosanase activity that is significantly higher than that of the conventional chitosanase. Furthermore, the surface hydration of liposomes used to prepare ICL that affected the activity of immobilized chitosanase verified the importance of liposome surfaces. Indeed, the stability of ICL assayed by measuring the chitosanase activity is significantly higher than that of conventional chitosanase under various temperatures and pH conditions. These characteristics of ICL show the possible preparation of the biocatalysts that can be prepared by immobilizing enzymes onto liposome vesicles properly.

元の言語English
ジャーナルJournal of Biotechnology
DOI
出版物ステータスAccepted/In press - 1800
外部発表Yes

    フィンガープリント

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology

これを引用

Ngo, K. X., Umakoshi, H., Shimanouchi, T., Sugaya, H., & Kuboi, R. (受理済み/印刷中). Chitosanase displayed on liposome can increase its activity and stability. Journal of Biotechnology. https://doi.org/10.1016/j.jbiotec.2010.01.014