@article{83d87dede31f4497b2f8454ce7908145,
title = "Circadian rhythmic changes of neuronal activity in the suprachiasmatic nucleus of the rat hypothalamic slice",
abstract = "The present study, utilizing rat brain slice of the suprachiasmatic nuclei (SCN) demonstrated single neuronal activity of SCN cells which changed in correspondence to environmental light-dark (L-D) schedules. The unit discharge rates of cells from animals kept on normal LL-D) cycles reached peaks at about 14.00 h and had a lowest point at around 02.00 h. The unit activity in slices from animals kept on reversed (L-D) cycles was shifted approximately 12 h.",
keywords = "brain slice, circadian rhythm, electrophysiology, rat, single unit activity, suprachiasmatic nucleus",
author = "Shigenobu Shibata and Yutaka Oomura and Hitoshi Kita and Kiichi Hattori",
note = "Funding Information: The entrainedn eurons mighset rvea s regulators of the many circadian relatefudn ctionso f the animals. This studyc learlyd emonstratethse abilityo f at leasts omeS CN neuronst o maintaint heircirca-dian rhythmf or somel imitedt imea fterd eafferenta-tion from the retinaa ndother braianr easin the ab-senceo f influencefr omm etaboliteasn dh ormoneisn the blood. Our resultsw, hichshow cleard ifferences between thleig ht and dark period activityo f SCN slicesi n in vitro conditionsc,o rrelatwe iththe results of studiesw ith 14C-labeled-2-deoxy-o-gluGcolus-e . coseu tilizationin the SCN is higherin the light than in the dark period,a ndthis differencies restrictetdo 1 Hendrickson, A. E., WagoNn.e arn, dC owanW, . M., An autoradiographainc de lectron microscopic sotuf dreyt i-no-hypothalamic connectZio. nZse,l lforsch.1, 35( 1972) 1-26. 2 Ibuka, Na. ndK awamura, H., Loosfs c ircadianrh ythm in sleep-wakefulnecsysc lein ther at by suprachiasmatic nucleus lesionBsra, in Research9, 6( 19757) 6-81. 3 IbukaN, ., NihonmatsuI., a ndS ekiguchSi,. , Sleep-wake-fulness rhythms in maifctee rs uprachiasrnatic nucleus lesionsW, akingS leeping,4 (19801) 67-173. 4 InouyeS, . T. andK awamuraH, ., Persistencoef c ircadian rhythmicity in a mammalian hypoth'iasllaamndicc' on-tainingt hes uprachiasmatic nucPleroucs., n at. Acad. Sci. U.S.A., 76( 19795) 962-5966. 5 Kita, H., ShibataS, ., Oomura, Y. and OoKk.i,, Excita-torye ffectos f the suprachiasmatic nuocnle thusev entro-medianl ucleuisn ther at hypothalamic sliBcrea,i n Re- the SCN 16,17. It has beenre portedth at SCN stimulationp ro-ducesm ainlye xcitatoryre sponseisn the ventromedial hypothalam5u,s and mainlyi nhibitory re-sponseisn the laterahly pothalamaicr eai n the rat1 2. This influenceo f the SCN in thesep articulahr ypo-thalamica reas,a nd other still uninvestigateadre as, may contributeto the control of circadianr hythm activity in animals. From the presentr esults,it might be suggestedth at environmentalilg ht pro-ducesu nknowns ubstancewsh ichi nterferew ithneu-ronal activityf or a long period, sincet he transmitters, per se, releasedfr omthe optic nervtee rminals would nots urvivea nd activaten eurons fosru cha long period.H oweveri,t has beenr eported th1a4t C-labeled-2-deoxy-D-glucuotsieli zationr hythm17 and multipleu nit dischargerh ythm4 in the SCN are not affectedb y ocular enucleationT.h is suggesttsh at the SCN may producei ts own intrinsic circadian oscillationO. ur experimeinntd icatesi,n any casea n importantr ole for the SCN in circadianr hythm organizationa,n dwe believteh ebrain slice method will prove usefula s a tool for betteru nderstanding the functionsa nd mechanismof circadianc locks, We thank Prof. A. Simpsonf or help in preparation of this manuscriptT.h is work was partly supported by Grant-in-Aid for Scientific Research 244021a nd 448103,a nd Grant-in-Aid foSrp ecial Project Research 212110, 3114084,2 1815 and 520967a, ndG rant-in-Aidfo r DevelopmentSacl ien-tific Research5 87035f rom the Ministry of Education, Sciencea nd Culture.",
year = "1982",
month = sep,
day = "9",
doi = "10.1016/0006-8993(82)91041-1",
language = "English",
volume = "247",
pages = "154--158",
journal = "Brain Research",
issn = "0006-8993",
number = "1",
}