Circularization of linear viroid RNA via 2′-phosphomonoester, 3′, 5′-phosphodiester bonds by a novel type of RNA ligase from wheat germ and Chlamydomonas

Yo Kikuchi*, Kazimierz Tyc, Witold Filipowicz, Heinz L. Sänger, Hans J. Gross

*この研究の対応する著者

研究成果: Article査読

51 被引用数 (Scopus)

抄録

A novel type of RNA ligase activity in extracts of wheat germ or Chlamydomonas requires 2′,3′-cyclic phosphate and 5′-phosphate ends for ligation to form a 2′-phosphomonoester, 3′,5′-phosphodiester bond. Using 5′-32P-labeled linear PSTV, we demonstrate that RNase T1-nicked viroid predominantly forms -G3′-P2′-P-5′U-bonds. Natural linear PSTV, however, forms main--G3′-P2′-P-5′A-bonds upon enzymatic circularization. We show that natural linear PSTV RNA has nicks between C181 and A182, or between C348 and A349, and that consequently C181 and C348 carry 2′,3′-cyclophosphate termini.

本文言語English
ページ(範囲)7521-7529
ページ数9
ジャーナルNucleic Acids Research
10
23
DOI
出版ステータスPublished - 1982 12 11
外部発表はい

ASJC Scopus subject areas

  • 統計学、確率および不確実性
  • 応用数学
  • 健康、毒物学および変異誘発
  • 毒物学
  • 遺伝学(臨床)
  • 遺伝学

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