Cleavage of tRNA within the mature tRNA sequence by the catalytic RNA of RNase P: Implication for the formation of the primer tRNA fragment for reverse transcription in copia retrovirus-like particles

Yo Kikuchi, Noriko Sasaki, Yumiko Ando-Yamagami

研究成果: Article査読

32 被引用数 (Scopus)

抄録

The retrovirus-like particles of Drosophila are intermediates of retrotransposition of the transposable element copia. In these particles, a 39-nucleotide-long fragment from the 5′ region of Drosophila initiator methionine tRNA (tRNAi Met) is used as the primer for copia minus-strand reverse transcription. To function as primer for this reverse transcription, the Drosophila tRNAi Met must be cleaved in vivo at the site between nucleotides 39 and 40. When a synthetic Drosophila tRNAi Met precursor was incubated with M1RNA, the catalytic RNA of Escherichia coli RNase P, other cleavages within the mature tRNA sequence were detected in addition to the efficient removal of the 5′ leader sequence of this tRNA precursor. One of these cleavage sites is between nucleotides 39 and 40 of Drosophila tRNAi Met. Based on this result, we propose a model for formation of the primer tRNA fragment for reverse transcription in copia retrovirus-like particles.

本文言語English
ページ(範囲)8105-8109
ページ数5
ジャーナルProceedings of the National Academy of Sciences of the United States of America
87
20
出版ステータスPublished - 1990
外部発表はい

ASJC Scopus subject areas

  • Genetics
  • General

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