Cloning and characterization of the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway genes of a natural-rubber producing plant, Hevea brasiliensis

Tomoki Sando, Shinya Takeno, Norie Watanabe, Hiroshi Okumoto, Tomohisa Kuzuyama, Atsushi Yamashita, Masahira Hattori, Naotake Ogasawara, Eiichiro Fukusaki, Akio Kobayashi

研究成果: Article

46 引用 (Scopus)

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Natural rubber is synthesized as rubber particles in the latex, the fluid cytoplasm of laticifers, of Hevea brasiliensis. Although it has been found that natural rubber is biosynthesized through the mevalonate pathway, the involvement of an alternative 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway is uncertain. We obtained all series of the MEP pathway candidate genes by analyzing expressed sequence tag (EST) information and degenerate PCR in H. brasiliensis. Complementation experiments with Escherichia coli mutants were performed to confirm the functions of the MEP pathway gene products of H. brasiliensis together with those of Arabidopsis thaliana, and it was found that 1-deoxy-D-xylulose-5-phosphate reductoisomerase, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, and 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase of H. brasiliensis were functionally active in the E. coli mutants. Gene expression analysis revealed that the expression level of the HbDXS2 gene in latex was relatively high as compared to those of other MEP pathway genes. However, a feeding experiment with [1-13C] 1-deoxy-D-xylulose triacetate, an intermediate derivative of the MEP pathway, indicated that the MEP pathway is not involved in rubber biosynthesis, but is involved in carotenoids biosynthesis in H. brasiliensis.

元の言語English
ページ(範囲)2903-2917
ページ数15
ジャーナルBioscience, Biotechnology and Biochemistry
72
発行部数11
DOI
出版物ステータスPublished - 2008
外部発表Yes

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ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

これを引用

Sando, T., Takeno, S., Watanabe, N., Okumoto, H., Kuzuyama, T., Yamashita, A., Hattori, M., Ogasawara, N., Fukusaki, E., & Kobayashi, A. (2008). Cloning and characterization of the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway genes of a natural-rubber producing plant, Hevea brasiliensis. Bioscience, Biotechnology and Biochemistry, 72(11), 2903-2917. https://doi.org/10.1271/bbb.80387