Cloning and characterization of the promoter of murine cytohesin-1 gene

Nobuhito Goda; Akito Tanoue; Shintaro Kikuchi; Gozoh Tsujimoto

doi:10.1016/S0167-4781(00)00160-3

Cloning and characterization of the promoter of murine cytohesin-1 gene

Nobuhito Goda, Akito Tanoue^*, Shintaro Kikuchi, Gozoh Tsujimoto

^*この研究の対応する著者

研究成果: Article › 査読

5 被引用数 (Scopus)

抄録

Mouse cytohesin-1 (CTH-1) is a guanine nucleotide exchange factor, specific for ADP ribosylation factors. The CTH-1 gene promoter was characterized by deletion mapping and mutational analysis. The region between -101 and -38 relative to the transcription start site showed the essential promoter activity when transfected into both NIH3T3 and COS7 cells. The nucleotides of the core promoter region contain three tandem GC boxes, which can offer potential binding sites for the ubiquitous transcription factor Sp- 1 family. Mutational analysis revealed that these tandem GC boxes are indispensable for activation of the gene transcription. (C) 2000 Elsevier Science B.V.

本文言語	English
ページ（範囲）	195-199
ページ数	5
ジャーナル	Biochimica et Biophysica Acta - Gene Structure and Expression
巻	1493
号	1-2
DOI	https://doi.org/10.1016/S0167-4781(00)00160-3
出版ステータス	Published - 2000 9月 7
外部発表	はい

ASJC Scopus subject areas

構造生物学
生物理学
生化学
遺伝学

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10.1016/S0167-4781(00)00160-3

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title = "Cloning and characterization of the promoter of murine cytohesin-1 gene",

abstract = "Mouse cytohesin-1 (CTH-1) is a guanine nucleotide exchange factor, specific for ADP ribosylation factors. The CTH-1 gene promoter was characterized by deletion mapping and mutational analysis. The region between -101 and -38 relative to the transcription start site showed the essential promoter activity when transfected into both NIH3T3 and COS7 cells. The nucleotides of the core promoter region contain three tandem GC boxes, which can offer potential binding sites for the ubiquitous transcription factor Sp- 1 family. Mutational analysis revealed that these tandem GC boxes are indispensable for activation of the gene transcription. (C) 2000 Elsevier Science B.V.",

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author = "Nobuhito Goda and Akito Tanoue and Shintaro Kikuchi and Gozoh Tsujimoto",

note = "Funding Information: Nobuhito Goda is a research fellow supported by the Japan Society for the Promotion of Science in 1999. This work was supported in part by research grants from the Scientific Fund of the Ministry of Education, Science, and Culture of Japan, the Japan Health Science Foundation and Ministry of Human Health and Welfare, the Organization for Pharmaceutical Safety and Research (OPSR), and a Grant for Liberal Harmonious Research Promotion System from the Science and Technology Agency.",

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AU - Tanoue, Akito

AU - Kikuchi, Shintaro

AU - Tsujimoto, Gozoh

N1 - Funding Information: Nobuhito Goda is a research fellow supported by the Japan Society for the Promotion of Science in 1999. This work was supported in part by research grants from the Scientific Fund of the Ministry of Education, Science, and Culture of Japan, the Japan Health Science Foundation and Ministry of Human Health and Welfare, the Organization for Pharmaceutical Safety and Research (OPSR), and a Grant for Liberal Harmonious Research Promotion System from the Science and Technology Agency.

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N2 - Mouse cytohesin-1 (CTH-1) is a guanine nucleotide exchange factor, specific for ADP ribosylation factors. The CTH-1 gene promoter was characterized by deletion mapping and mutational analysis. The region between -101 and -38 relative to the transcription start site showed the essential promoter activity when transfected into both NIH3T3 and COS7 cells. The nucleotides of the core promoter region contain three tandem GC boxes, which can offer potential binding sites for the ubiquitous transcription factor Sp- 1 family. Mutational analysis revealed that these tandem GC boxes are indispensable for activation of the gene transcription. (C) 2000 Elsevier Science B.V.

AB - Mouse cytohesin-1 (CTH-1) is a guanine nucleotide exchange factor, specific for ADP ribosylation factors. The CTH-1 gene promoter was characterized by deletion mapping and mutational analysis. The region between -101 and -38 relative to the transcription start site showed the essential promoter activity when transfected into both NIH3T3 and COS7 cells. The nucleotides of the core promoter region contain three tandem GC boxes, which can offer potential binding sites for the ubiquitous transcription factor Sp- 1 family. Mutational analysis revealed that these tandem GC boxes are indispensable for activation of the gene transcription. (C) 2000 Elsevier Science B.V.

KW - Cytohesin-1

KW - Murine

KW - Promoter

KW - Sp-1

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Cloning and characterization of the promoter of murine cytohesin-1 gene

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