Cloning and nucleotide sequencing of two insecticidal δ- endotoxin genes from bacillus thuringiensis var. Kurstaki hd-1 dna

Shin Ichi Kondo, Nobuya Tamura, Akane Kunitate, Masahira Hattori, Akira Akashi, Iwao Ohmori

研究成果: Article査読

29 被引用数 (Scopus)

抄録

The insecticidal δ-endotoxin genes cry-1-1 and cry-1-2 derived from Bacillus thuringiensis var. kurstaki HD-1 were cloned, and their nucleotide sequences were determined. The nucleotide sequence of cry-1-1 was identical with the one reported by Schnepf et al. except for one base in the coding region. The coding regions of our two cloned toxin genes were not identical, but showed homology. More intensive variations in the sequences were observed in some regions, predominantly in the center of the coding region, 3’-terminal portion and 3-flanking region. The differences in nucleotide sequences may have been mainly induced by base exchanges and deletions. Both translated products in E. coli were toxic to Bombyx mori larvae, but their insecticidal activities were considerably different.

本文言語English
ページ(範囲)455-463
ページ数9
ジャーナルAgricultural and Biological Chemistry
51
2
DOI
出版ステータスPublished - 1987
外部発表はい

ASJC Scopus subject areas

  • 農業および生物科学(全般)
  • 生化学、遺伝学、分子生物学(全般)

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