Cloning and sequencing of the cDNA encoding lipase I from Trichosporon fermentans WU-C12

Tsuyoshi Arai, Satoshi Yusa, Kotaro Kirimura, Shoji Usami

    研究成果: Article

    6 引用 (Scopus)

    抄録

    A cDNA clone encoding extracellular lipase I (TFL I) from Trichosporon fermentans WU-C12 was isolated and characterized. The TFL I cDNA was isolated from a λ gt 10-based cDNA library using as a probe a 0.8 kb fragment, amplified by PCR with synthetic oligonucleotide corresponding to the partial amino acid sequences of TFL I. The cDNA encodes a protein consisting of 563 amino acids containing a putative signal peptide of 19 amino acids. The deduced amino acid sequence shares 99.5%, overall identity with that of lipase II (GCL II) from Geotrichum candidum ATCC 34614, whereas TFL I is a trimer enzyme and GCL II monomer. Southern hybridization with the TFL I cDNA as a probe revealed that WU-C12 contained two different lipase genes.

    元の言語English
    ページ(範囲)183-188
    ページ数6
    ジャーナルFEMS Microbiology Letters
    152
    発行部数1
    DOI
    出版物ステータスPublished - 1997 7 1

    Fingerprint

    Trichosporon
    Lipase
    Organism Cloning
    Complementary DNA
    Amino Acid Sequence
    Geotrichum
    Amino Acids
    Protein Sorting Signals
    Gene Library
    Oligonucleotides
    Clone Cells
    Polymerase Chain Reaction
    Enzymes
    Genes
    Proteins

    ASJC Scopus subject areas

    • Genetics
    • Molecular Biology
    • Applied Microbiology and Biotechnology
    • Microbiology

    これを引用

    Cloning and sequencing of the cDNA encoding lipase I from Trichosporon fermentans WU-C12. / Arai, Tsuyoshi; Yusa, Satoshi; Kirimura, Kotaro; Usami, Shoji.

    :: FEMS Microbiology Letters, 巻 152, 番号 1, 01.07.1997, p. 183-188.

    研究成果: Article

    Arai, Tsuyoshi ; Yusa, Satoshi ; Kirimura, Kotaro ; Usami, Shoji. / Cloning and sequencing of the cDNA encoding lipase I from Trichosporon fermentans WU-C12. :: FEMS Microbiology Letters. 1997 ; 巻 152, 番号 1. pp. 183-188.
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