Cloning and sequencing of the cDNA encoding lipase I from Trichosporon fermentans WU-C12

Tsuyoshi Arai, Satoshi Yusa, Kohtaro Kirimura*, Shoji Usami

*この研究の対応する著者

研究成果査読

6 被引用数 (Scopus)

抄録

A cDNA clone encoding extracellular lipase I (TFL I) from Trichosporon fermentans WU-C12 was isolated and characterized. The TFL I cDNA was isolated from a λ gt 10-based cDNA library using as a probe a 0.8 kb fragment, amplified by PCR with synthetic oligonucleotide corresponding to the partial amino acid sequences of TFL I. The cDNA encodes a protein consisting of 563 amino acids containing a putative signal peptide of 19 amino acids. The deduced amino acid sequence shares 99.5%, overall identity with that of lipase II (GCL II) from Geotrichum candidum ATCC 34614, whereas TFL I is a trimer enzyme and GCL II monomer. Southern hybridization with the TFL I cDNA as a probe revealed that WU-C12 contained two different lipase genes.

本文言語English
ページ(範囲)183-188
ページ数6
ジャーナルFEMS Microbiology Letters
152
1
DOI
出版ステータスPublished - 1997 7 1

ASJC Scopus subject areas

  • 微生物学
  • 分子生物学
  • 遺伝学

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