Cloning of mouse BMAL2 and its daily expression profile in the suprachiasmatic nucleus: A remarkable acceleration of Bmal2 sequence divergence after Bmal gene duplication

Toshiyuki Okano, Momoko Sasaki, Yoshitaka Fukada*

*この研究の対応する著者

研究成果: Article査読

36 被引用数 (Scopus)

抄録

Brain-Muscle-Arnt-Like-protein 2 (BMAL2; Arnt4) (aryl hydrocarbon receptor nuclear translocator) is a recently identified basic Helix-Loop-Helix-Per-Arnt-Sim (bHLH-PAS) transcription factor, which contributes to a positive regulation of autoregulatory feedback loop in vertebrate circadian clock systems. In this study, we cloned cDNAs encoding mouse and rat BMAL2 (mBMAL2 and rBMAL2) from mouse midbrain and rat-1 fibroblast cells, respectively. A phylogenetic analysis strongly suggested that vertebrate Bmal1 and Bmal2 genes were generated by a single gene duplication of an ancestral Bmal gene, a vertebrate ortholog of dCyc gene, and that 'BMAL2's putatively termed so far are orthologous. Interestingly, BMAL2 proteins have diverged about 20-fold more rapidly than BMAL1 proteins after the duplication, suggesting an as-yet-unidentified function conserved in BMAL1 but not in BMAL2. mBmal2 mRNA was constitutively expressed throughout the day under light-dark cycle in the mouse hypothalamus containing suprachiasmatic nucleus, the site of the central circadian oscillator in mammals.

本文言語English
ページ(範囲)111-114
ページ数4
ジャーナルNeuroscience Letters
300
2
DOI
出版ステータスPublished - 2001 3月 9
外部発表はい

ASJC Scopus subject areas

  • 神経科学(全般)

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