Coexpression of thrombopoietin and c-mpl genes in human acute myeloblastic leukemia cells

I. Matsumura, Y. Kanakura, H. Ikeda, J. Ishikawa, H. Yoshida, Y. Horikawa, T. Nishiura, T. Tahara, Takashi Kato, H. Miyazaki, Y. Matsuzawa

研究成果: Article

26 引用 (Scopus)

抄録

Thrombopoietin (TPO) is a recently identified hematopoietic growth factor that is essential for the growth and development of megakaryocytes. We have previously shown that TPO induces proliferation of acute myeloblastic leukemia (AML) cells in vitro. In this study, we have examined the expression of TPO and its receptor c-mpl in a series of AML cases and human leukemia cell lines. The mRNA transcripts of TPO were detectable in 18 of 50 AML cases and in some myeloid leukemia cell lines (HEL, M07E and CMK) by means of reverse transcriptase polymerase chain reaction (RT-PCR). In addition, TPO transcripts were coexpressed with c-mpl transcripts in 10 of 50 AML cases and in HEL, M07E and CMK cells. With regard to the French-American-British (FAB) classification, coexpression of TPO and c-mpl was observed with high frequency in AML cases of M7-type. Despite the TPO expression in a substantial fraction of leukemia cells, biological activity of TPO was not found in the conditioned medium that was obtained from cultivation of TPO mRNA-positive leukemia cells. These results suggest that TPO may not commonly participate in the abnormal growth of AML cells as an extracellular autocrine growth factor.

元の言語English
ページ(範囲)91-94
ページ数4
ジャーナルLeukemia
10
発行部数1
出版物ステータスPublished - 1996 1
外部発表Yes

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Thrombopoietin
Acute Myeloid Leukemia
Genes
Leukemia
Intercellular Signaling Peptides and Proteins
Thrombopoietin Receptors
Cell Line
Messenger RNA
Myeloid Leukemia
Megakaryocytes
Myeloid Cells
Conditioned Culture Medium
Reverse Transcriptase Polymerase Chain Reaction
Growth and Development

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

これを引用

Matsumura, I., Kanakura, Y., Ikeda, H., Ishikawa, J., Yoshida, H., Horikawa, Y., ... Matsuzawa, Y. (1996). Coexpression of thrombopoietin and c-mpl genes in human acute myeloblastic leukemia cells. Leukemia, 10(1), 91-94.

Coexpression of thrombopoietin and c-mpl genes in human acute myeloblastic leukemia cells. / Matsumura, I.; Kanakura, Y.; Ikeda, H.; Ishikawa, J.; Yoshida, H.; Horikawa, Y.; Nishiura, T.; Tahara, T.; Kato, Takashi; Miyazaki, H.; Matsuzawa, Y.

:: Leukemia, 巻 10, 番号 1, 01.1996, p. 91-94.

研究成果: Article

Matsumura, I, Kanakura, Y, Ikeda, H, Ishikawa, J, Yoshida, H, Horikawa, Y, Nishiura, T, Tahara, T, Kato, T, Miyazaki, H & Matsuzawa, Y 1996, 'Coexpression of thrombopoietin and c-mpl genes in human acute myeloblastic leukemia cells', Leukemia, 巻. 10, 番号 1, pp. 91-94.
Matsumura I, Kanakura Y, Ikeda H, Ishikawa J, Yoshida H, Horikawa Y その他. Coexpression of thrombopoietin and c-mpl genes in human acute myeloblastic leukemia cells. Leukemia. 1996 1;10(1):91-94.
Matsumura, I. ; Kanakura, Y. ; Ikeda, H. ; Ishikawa, J. ; Yoshida, H. ; Horikawa, Y. ; Nishiura, T. ; Tahara, T. ; Kato, Takashi ; Miyazaki, H. ; Matsuzawa, Y. / Coexpression of thrombopoietin and c-mpl genes in human acute myeloblastic leukemia cells. :: Leukemia. 1996 ; 巻 10, 番号 1. pp. 91-94.
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abstract = "Thrombopoietin (TPO) is a recently identified hematopoietic growth factor that is essential for the growth and development of megakaryocytes. We have previously shown that TPO induces proliferation of acute myeloblastic leukemia (AML) cells in vitro. In this study, we have examined the expression of TPO and its receptor c-mpl in a series of AML cases and human leukemia cell lines. The mRNA transcripts of TPO were detectable in 18 of 50 AML cases and in some myeloid leukemia cell lines (HEL, M07E and CMK) by means of reverse transcriptase polymerase chain reaction (RT-PCR). In addition, TPO transcripts were coexpressed with c-mpl transcripts in 10 of 50 AML cases and in HEL, M07E and CMK cells. With regard to the French-American-British (FAB) classification, coexpression of TPO and c-mpl was observed with high frequency in AML cases of M7-type. Despite the TPO expression in a substantial fraction of leukemia cells, biological activity of TPO was not found in the conditioned medium that was obtained from cultivation of TPO mRNA-positive leukemia cells. These results suggest that TPO may not commonly participate in the abnormal growth of AML cells as an extracellular autocrine growth factor.",
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AU - Matsumura, I.

AU - Kanakura, Y.

AU - Ikeda, H.

AU - Ishikawa, J.

AU - Yoshida, H.

AU - Horikawa, Y.

AU - Nishiura, T.

AU - Tahara, T.

AU - Kato, Takashi

AU - Miyazaki, H.

AU - Matsuzawa, Y.

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