Colony fingerprint for discrimination of microbial species based on lensless imaging of microcolonies

Yoshiaki Maeda, Hironori Dobashi, Yui Sugiyama, Tatsuya Saeki, Tae Kyu Lim, Manabu Harada, Tadashi Matsunaga, Tomoko Yoshino, Tsuyoshi Tanaka

研究成果: Article

8 引用 (Scopus)

抜粋

Detection and identification of microbial species are crucial in a wide range of industries, including production of beverages, foods, cosmetics, and pharmaceuticals. Traditionally, colony formation and its morphological analysis (e.g., size, shape, and color) with a naked eye have been employed for this purpose. However, such a conventional method is time consuming, labor intensive, and not very reproducible. To overcome these problems, we propose a novel method that detects microcolonies (diameter 10-500 μm) using a lensless imaging system. When comparing colony images of five microorganisms from different genera (Escherichia coli, Salmonella enterica, Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans), the images showed obvious different features. Being closely related species, St. aureus and St. epidermidis resembled each other, but the imaging analysis could extract substantial information (colony fingerprints) including the morphological and physiological features, and linear discriminant analysis of the colony fingerprints distinguished these two species with 100% of accuracy. Because this system may offer many advantages such as high-throughput testing, lower costs, more compact equipment, and ease of automation, it holds promise for microbial detection and identification in various academic and industrial areas.

元の言語English
記事番号e0174723
ジャーナルPLoS One
12
発行部数4
DOI
出版物ステータスPublished - 2017 3 1
外部発表Yes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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  • これを引用

    Maeda, Y., Dobashi, H., Sugiyama, Y., Saeki, T., Lim, T. K., Harada, M., Matsunaga, T., Yoshino, T., & Tanaka, T. (2017). Colony fingerprint for discrimination of microbial species based on lensless imaging of microcolonies. PLoS One, 12(4), [e0174723]. https://doi.org/10.1371/journal.pone.0174723