Comparative genome analysis of three eukaryotic parasites with differing abilities to transform leukocytes reveals key mediators of Theileria-induced leukocyte transformation.

Kyoko Hayashida, Yuichiro Hara, Takashi Abe, Chisato Yamasaki, Atsushi Toyoda, Takehide Kosuge, Yutaka Suzuki, Yoshiharu Sato, Shuichi Kawashima, Toshiaki Katayama, Hiroyuki Wakaguri, Noboru Inoue, Keiichi Homma, Masahito Tada-Umezaki, Yukio Yagi, Yasuyuki Fujii, Takuya Habara, Minoru Kanehisa, Hidemi Watanabe, Kimihito ItoTakashi Gojobori, Hideaki Sugawara, Tadashi Imanishi, William Weir, Malcolm Gardner, Arnab Pain, Brian Shiels, Masahira Hattori, Vishvanath Nene, Chihiro Sugimoto

研究成果: Article

抄録

We sequenced the genome of Theileria orientalis, a tick-borne apicomplexan protozoan parasite of cattle. The focus of this study was a comparative genome analysis of T. orientalis relative to other highly pathogenic Theileria species, T. parva and T. annulata. T. parva and T. annulata induce transformation of infected cells of lymphocyte or macrophage/monocyte lineages; in contrast, T. orientalis does not induce uncontrolled proliferation of infected leukocytes and multiplies predominantly within infected erythrocytes. While synteny across homologous chromosomes of the three Theileria species was found to be well conserved overall, subtelomeric structures were found to differ substantially, as T. orientalis lacks the large tandemly arrayed subtelomere-encoded variable secreted protein-encoding gene family. Moreover, expansion of particular gene families by gene duplication was found in the genomes of the two transforming Theileria species, most notably, the TashAT/TpHN and Tar/Tpr gene families. Gene families that are present only in T. parva and T. annulata and not in T. orientalis, Babesia bovis, or Plasmodium were also identified. Identification of differences between the genome sequences of Theileria species with different abilities to transform and immortalize bovine leukocytes will provide insight into proteins and mechanisms that have evolved to induce and regulate this process. The T. orientalis genome database is available at http://totdb.czc.hokudai.ac.jp/.

元の言語English
ジャーナルmBio
3
発行部数5
出版物ステータスPublished - 2012
外部発表Yes

Fingerprint

Theileria
Parasites
Leukocytes
Genome
Babesia bovis
Genes
Synteny
Tars
Gene Duplication
Plasmodium
Ticks
Monocytes
Proteins
Chromosomes
Erythrocytes
Macrophages
Databases
Lymphocytes

ASJC Scopus subject areas

  • Microbiology
  • Virology

これを引用

Comparative genome analysis of three eukaryotic parasites with differing abilities to transform leukocytes reveals key mediators of Theileria-induced leukocyte transformation. / Hayashida, Kyoko; Hara, Yuichiro; Abe, Takashi; Yamasaki, Chisato; Toyoda, Atsushi; Kosuge, Takehide; Suzuki, Yutaka; Sato, Yoshiharu; Kawashima, Shuichi; Katayama, Toshiaki; Wakaguri, Hiroyuki; Inoue, Noboru; Homma, Keiichi; Tada-Umezaki, Masahito; Yagi, Yukio; Fujii, Yasuyuki; Habara, Takuya; Kanehisa, Minoru; Watanabe, Hidemi; Ito, Kimihito; Gojobori, Takashi; Sugawara, Hideaki; Imanishi, Tadashi; Weir, William; Gardner, Malcolm; Pain, Arnab; Shiels, Brian; Hattori, Masahira; Nene, Vishvanath; Sugimoto, Chihiro.

:: mBio, 巻 3, 番号 5, 2012.

研究成果: Article

Hayashida, K, Hara, Y, Abe, T, Yamasaki, C, Toyoda, A, Kosuge, T, Suzuki, Y, Sato, Y, Kawashima, S, Katayama, T, Wakaguri, H, Inoue, N, Homma, K, Tada-Umezaki, M, Yagi, Y, Fujii, Y, Habara, T, Kanehisa, M, Watanabe, H, Ito, K, Gojobori, T, Sugawara, H, Imanishi, T, Weir, W, Gardner, M, Pain, A, Shiels, B, Hattori, M, Nene, V & Sugimoto, C 2012, 'Comparative genome analysis of three eukaryotic parasites with differing abilities to transform leukocytes reveals key mediators of Theileria-induced leukocyte transformation.', mBio, 巻. 3, 番号 5.
Hayashida, Kyoko ; Hara, Yuichiro ; Abe, Takashi ; Yamasaki, Chisato ; Toyoda, Atsushi ; Kosuge, Takehide ; Suzuki, Yutaka ; Sato, Yoshiharu ; Kawashima, Shuichi ; Katayama, Toshiaki ; Wakaguri, Hiroyuki ; Inoue, Noboru ; Homma, Keiichi ; Tada-Umezaki, Masahito ; Yagi, Yukio ; Fujii, Yasuyuki ; Habara, Takuya ; Kanehisa, Minoru ; Watanabe, Hidemi ; Ito, Kimihito ; Gojobori, Takashi ; Sugawara, Hideaki ; Imanishi, Tadashi ; Weir, William ; Gardner, Malcolm ; Pain, Arnab ; Shiels, Brian ; Hattori, Masahira ; Nene, Vishvanath ; Sugimoto, Chihiro. / Comparative genome analysis of three eukaryotic parasites with differing abilities to transform leukocytes reveals key mediators of Theileria-induced leukocyte transformation. :: mBio. 2012 ; 巻 3, 番号 5.
@article{37f64fd85938451bb7fb208b08e4f3cf,
title = "Comparative genome analysis of three eukaryotic parasites with differing abilities to transform leukocytes reveals key mediators of Theileria-induced leukocyte transformation.",
abstract = "We sequenced the genome of Theileria orientalis, a tick-borne apicomplexan protozoan parasite of cattle. The focus of this study was a comparative genome analysis of T. orientalis relative to other highly pathogenic Theileria species, T. parva and T. annulata. T. parva and T. annulata induce transformation of infected cells of lymphocyte or macrophage/monocyte lineages; in contrast, T. orientalis does not induce uncontrolled proliferation of infected leukocytes and multiplies predominantly within infected erythrocytes. While synteny across homologous chromosomes of the three Theileria species was found to be well conserved overall, subtelomeric structures were found to differ substantially, as T. orientalis lacks the large tandemly arrayed subtelomere-encoded variable secreted protein-encoding gene family. Moreover, expansion of particular gene families by gene duplication was found in the genomes of the two transforming Theileria species, most notably, the TashAT/TpHN and Tar/Tpr gene families. Gene families that are present only in T. parva and T. annulata and not in T. orientalis, Babesia bovis, or Plasmodium were also identified. Identification of differences between the genome sequences of Theileria species with different abilities to transform and immortalize bovine leukocytes will provide insight into proteins and mechanisms that have evolved to induce and regulate this process. The T. orientalis genome database is available at http://totdb.czc.hokudai.ac.jp/.",
author = "Kyoko Hayashida and Yuichiro Hara and Takashi Abe and Chisato Yamasaki and Atsushi Toyoda and Takehide Kosuge and Yutaka Suzuki and Yoshiharu Sato and Shuichi Kawashima and Toshiaki Katayama and Hiroyuki Wakaguri and Noboru Inoue and Keiichi Homma and Masahito Tada-Umezaki and Yukio Yagi and Yasuyuki Fujii and Takuya Habara and Minoru Kanehisa and Hidemi Watanabe and Kimihito Ito and Takashi Gojobori and Hideaki Sugawara and Tadashi Imanishi and William Weir and Malcolm Gardner and Arnab Pain and Brian Shiels and Masahira Hattori and Vishvanath Nene and Chihiro Sugimoto",
year = "2012",
language = "English",
volume = "3",
journal = "mBio",
issn = "2161-2129",
publisher = "American Society for Microbiology",
number = "5",

}

TY - JOUR

T1 - Comparative genome analysis of three eukaryotic parasites with differing abilities to transform leukocytes reveals key mediators of Theileria-induced leukocyte transformation.

AU - Hayashida, Kyoko

AU - Hara, Yuichiro

AU - Abe, Takashi

AU - Yamasaki, Chisato

AU - Toyoda, Atsushi

AU - Kosuge, Takehide

AU - Suzuki, Yutaka

AU - Sato, Yoshiharu

AU - Kawashima, Shuichi

AU - Katayama, Toshiaki

AU - Wakaguri, Hiroyuki

AU - Inoue, Noboru

AU - Homma, Keiichi

AU - Tada-Umezaki, Masahito

AU - Yagi, Yukio

AU - Fujii, Yasuyuki

AU - Habara, Takuya

AU - Kanehisa, Minoru

AU - Watanabe, Hidemi

AU - Ito, Kimihito

AU - Gojobori, Takashi

AU - Sugawara, Hideaki

AU - Imanishi, Tadashi

AU - Weir, William

AU - Gardner, Malcolm

AU - Pain, Arnab

AU - Shiels, Brian

AU - Hattori, Masahira

AU - Nene, Vishvanath

AU - Sugimoto, Chihiro

PY - 2012

Y1 - 2012

N2 - We sequenced the genome of Theileria orientalis, a tick-borne apicomplexan protozoan parasite of cattle. The focus of this study was a comparative genome analysis of T. orientalis relative to other highly pathogenic Theileria species, T. parva and T. annulata. T. parva and T. annulata induce transformation of infected cells of lymphocyte or macrophage/monocyte lineages; in contrast, T. orientalis does not induce uncontrolled proliferation of infected leukocytes and multiplies predominantly within infected erythrocytes. While synteny across homologous chromosomes of the three Theileria species was found to be well conserved overall, subtelomeric structures were found to differ substantially, as T. orientalis lacks the large tandemly arrayed subtelomere-encoded variable secreted protein-encoding gene family. Moreover, expansion of particular gene families by gene duplication was found in the genomes of the two transforming Theileria species, most notably, the TashAT/TpHN and Tar/Tpr gene families. Gene families that are present only in T. parva and T. annulata and not in T. orientalis, Babesia bovis, or Plasmodium were also identified. Identification of differences between the genome sequences of Theileria species with different abilities to transform and immortalize bovine leukocytes will provide insight into proteins and mechanisms that have evolved to induce and regulate this process. The T. orientalis genome database is available at http://totdb.czc.hokudai.ac.jp/.

AB - We sequenced the genome of Theileria orientalis, a tick-borne apicomplexan protozoan parasite of cattle. The focus of this study was a comparative genome analysis of T. orientalis relative to other highly pathogenic Theileria species, T. parva and T. annulata. T. parva and T. annulata induce transformation of infected cells of lymphocyte or macrophage/monocyte lineages; in contrast, T. orientalis does not induce uncontrolled proliferation of infected leukocytes and multiplies predominantly within infected erythrocytes. While synteny across homologous chromosomes of the three Theileria species was found to be well conserved overall, subtelomeric structures were found to differ substantially, as T. orientalis lacks the large tandemly arrayed subtelomere-encoded variable secreted protein-encoding gene family. Moreover, expansion of particular gene families by gene duplication was found in the genomes of the two transforming Theileria species, most notably, the TashAT/TpHN and Tar/Tpr gene families. Gene families that are present only in T. parva and T. annulata and not in T. orientalis, Babesia bovis, or Plasmodium were also identified. Identification of differences between the genome sequences of Theileria species with different abilities to transform and immortalize bovine leukocytes will provide insight into proteins and mechanisms that have evolved to induce and regulate this process. The T. orientalis genome database is available at http://totdb.czc.hokudai.ac.jp/.

UR - http://www.scopus.com/inward/record.url?scp=84872249517&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872249517&partnerID=8YFLogxK

M3 - Article

C2 - 22951932

VL - 3

JO - mBio

JF - mBio

SN - 2161-2129

IS - 5

ER -