Complete genome sequence and comparative analysis of the wild-type commensal escherichia coli strain se11 isolated from a healthy adult

Kenshiro Oshima, Hidehiro Toh, Yoshitoshi Ogura, Hiroyuki Sasamoto, Hidetoshi Morita, Sang Hee Park, Tadasuke Ooka, Sunao Iyoda, Todd D. Taylor, Tetsuya Hayashi, Kikuji Itoh, Masahira Hattori

研究成果: Article

55 引用 (Scopus)

抄録

We sequenced and analyzed the genome of a commensal Escherichia coli (E. coli) strain SE11 (O152:H28) recently isolated from feces of a healthy adult and classified into E. coli phylogenetic group B1. SE11 harbored a 4.8 Mb chromosome encoding 4679 protein-coding genes and six plasmids encoding 323 protein-coding genes. None of the SE11 genes had sequence similarity to known genes encoding phage- and plasmid-borne virulence factors found in pathogenic E. coli strains. The comparative genome analysis with the laboratory strain K-12 MG1655 identified 62 poorly conserved genes between these two non-pathogenic strains and 1186 genes absent in MG1655. These genes in SE11 were mostly encoded in large insertion regions on the chromosome or in the plasmids, and were notably abundant in genes of fimbriae and autotransporters, which are cell surface appendages that largely contribute to the adherence ability of bacteria to host cells and bacterial conjugation. These data suggest that SE11 may have evolved to acquire and accumulate the functions advantageous for stable colonization of intestinal cells, and that the adhesion-associated functions are important for the commensality of E. coli in human gut habitat.

元の言語English
ページ(範囲)375-386
ページ数12
ジャーナルDNA Research
15
発行部数6
DOI
出版物ステータスPublished - 2008 12
外部発表Yes

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Sequence Analysis
Genome
Escherichia coli
Genes
Plasmids
Genetic Conjugation
Chromosomes
Virulence Factors
Cell Adhesion
Feces
Bacteriophages
Ecosystem
Proteins
Bacteria

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Medicine(all)

これを引用

Complete genome sequence and comparative analysis of the wild-type commensal escherichia coli strain se11 isolated from a healthy adult. / Oshima, Kenshiro; Toh, Hidehiro; Ogura, Yoshitoshi; Sasamoto, Hiroyuki; Morita, Hidetoshi; Park, Sang Hee; Ooka, Tadasuke; Iyoda, Sunao; Taylor, Todd D.; Hayashi, Tetsuya; Itoh, Kikuji; Hattori, Masahira.

:: DNA Research, 巻 15, 番号 6, 12.2008, p. 375-386.

研究成果: Article

Oshima, K, Toh, H, Ogura, Y, Sasamoto, H, Morita, H, Park, SH, Ooka, T, Iyoda, S, Taylor, TD, Hayashi, T, Itoh, K & Hattori, M 2008, 'Complete genome sequence and comparative analysis of the wild-type commensal escherichia coli strain se11 isolated from a healthy adult', DNA Research, 巻. 15, 番号 6, pp. 375-386. https://doi.org/10.1093/dnares/dsn026
Oshima, Kenshiro ; Toh, Hidehiro ; Ogura, Yoshitoshi ; Sasamoto, Hiroyuki ; Morita, Hidetoshi ; Park, Sang Hee ; Ooka, Tadasuke ; Iyoda, Sunao ; Taylor, Todd D. ; Hayashi, Tetsuya ; Itoh, Kikuji ; Hattori, Masahira. / Complete genome sequence and comparative analysis of the wild-type commensal escherichia coli strain se11 isolated from a healthy adult. :: DNA Research. 2008 ; 巻 15, 番号 6. pp. 375-386.
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abstract = "We sequenced and analyzed the genome of a commensal Escherichia coli (E. coli) strain SE11 (O152:H28) recently isolated from feces of a healthy adult and classified into E. coli phylogenetic group B1. SE11 harbored a 4.8 Mb chromosome encoding 4679 protein-coding genes and six plasmids encoding 323 protein-coding genes. None of the SE11 genes had sequence similarity to known genes encoding phage- and plasmid-borne virulence factors found in pathogenic E. coli strains. The comparative genome analysis with the laboratory strain K-12 MG1655 identified 62 poorly conserved genes between these two non-pathogenic strains and 1186 genes absent in MG1655. These genes in SE11 were mostly encoded in large insertion regions on the chromosome or in the plasmids, and were notably abundant in genes of fimbriae and autotransporters, which are cell surface appendages that largely contribute to the adherence ability of bacteria to host cells and bacterial conjugation. These data suggest that SE11 may have evolved to acquire and accumulate the functions advantageous for stable colonization of intestinal cells, and that the adhesion-associated functions are important for the commensality of E. coli in human gut habitat.",
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AU - Sasamoto, Hiroyuki

AU - Morita, Hidetoshi

AU - Park, Sang Hee

AU - Ooka, Tadasuke

AU - Iyoda, Sunao

AU - Taylor, Todd D.

AU - Hayashi, Tetsuya

AU - Itoh, Kikuji

AU - Hattori, Masahira

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