抄録
The enterohemorrhagic Escherichia coli (EHEC) 0157:H7 strain RIMD 0509952, derived from an outbreak in Sakai city, Japan, in 1996, produces two kinds of verotoxins, VT1 and VT2, encoded by the stx1 and stx2 genes. In the EHEC strains, as well as in other VT-producing E. coli strains, the toxins are encoded by lysogenic bacteriophages. The EHEC 0157:H7 strain RIMD 0509952 did not produce plaque-forming phage particles upon inducing treatments. We have determined the complete nucleotide sequence of a prophage, VT2-Sakai, carrying the stx2A and stx2B genes on the chromosome, and presumed the putative functions of the encoded proteins and the cis-acting DNA elements based on sequence homology data. To our surprise, the sequences in the regions of VT2-Sakai corresponding to the early gene regulators and replication proteins, and the DNA sequences recognized by the regulators share very limited homology to those of the VT2-encoding 933W phage carried by the EHEC 0157:H7 strain EDL933 reported by Plunkett et al. (J. Bacteriol., p1767-1778, 181, 1999), although the sequences corresponding to the structural components are almost identical. These data suggest that these two phages were derived from a common ancestral phage and that either or both of them underwent multiple genetic rearrangements. An IS629 insertion was found downstream of the stx2B gene and upstream of the lysis gene S, and this might be responsible for the absence of plaque-forming activity in the lysate obtained after inducing treatments.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 227-239 |
| ページ数 | 13 |
| ジャーナル | Genes and Genetic Systems |
| 巻 | 74 |
| 発行部数 | 5 |
| DOI | |
| 出版物ステータス | Published - 1999 10 |
| 外部発表 | Yes |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Genetics
- Genetics(clinical)
これを引用
Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157 : H7 derived from the Sakai outbreak. / Makino, Kozo; Yokoyama, Katsushi; Kubota, Yoshino; Yutsudo, Chikako H.; Kimura, Sigenobu; Kurokawa, Ken; Ishii, Kazuo; Hattori, Masahira; Tatsuno, Ichiro; Abe, Hiroyuki; Iida, Tetsuya; Yamamoto, Koichiro; Onishi, Makoto; Hayashi, Tetsuya; Yasunaga, Teruo; Honda, Takeshi; Sasakawa, Chihiro; Shinagawa, Hideo.
：: Genes and Genetic Systems, 巻 74, 番号 5, 10.1999, p. 227-239.研究成果: Article
}
TY - JOUR
T1 - Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157
T2 - H7 derived from the Sakai outbreak
AU - Makino, Kozo
AU - Yokoyama, Katsushi
AU - Kubota, Yoshino
AU - Yutsudo, Chikako H.
AU - Kimura, Sigenobu
AU - Kurokawa, Ken
AU - Ishii, Kazuo
AU - Hattori, Masahira
AU - Tatsuno, Ichiro
AU - Abe, Hiroyuki
AU - Iida, Tetsuya
AU - Yamamoto, Koichiro
AU - Onishi, Makoto
AU - Hayashi, Tetsuya
AU - Yasunaga, Teruo
AU - Honda, Takeshi
AU - Sasakawa, Chihiro
AU - Shinagawa, Hideo
PY - 1999/10
Y1 - 1999/10
N2 - The enterohemorrhagic Escherichia coli (EHEC) 0157:H7 strain RIMD 0509952, derived from an outbreak in Sakai city, Japan, in 1996, produces two kinds of verotoxins, VT1 and VT2, encoded by the stx1 and stx2 genes. In the EHEC strains, as well as in other VT-producing E. coli strains, the toxins are encoded by lysogenic bacteriophages. The EHEC 0157:H7 strain RIMD 0509952 did not produce plaque-forming phage particles upon inducing treatments. We have determined the complete nucleotide sequence of a prophage, VT2-Sakai, carrying the stx2A and stx2B genes on the chromosome, and presumed the putative functions of the encoded proteins and the cis-acting DNA elements based on sequence homology data. To our surprise, the sequences in the regions of VT2-Sakai corresponding to the early gene regulators and replication proteins, and the DNA sequences recognized by the regulators share very limited homology to those of the VT2-encoding 933W phage carried by the EHEC 0157:H7 strain EDL933 reported by Plunkett et al. (J. Bacteriol., p1767-1778, 181, 1999), although the sequences corresponding to the structural components are almost identical. These data suggest that these two phages were derived from a common ancestral phage and that either or both of them underwent multiple genetic rearrangements. An IS629 insertion was found downstream of the stx2B gene and upstream of the lysis gene S, and this might be responsible for the absence of plaque-forming activity in the lysate obtained after inducing treatments.
AB - The enterohemorrhagic Escherichia coli (EHEC) 0157:H7 strain RIMD 0509952, derived from an outbreak in Sakai city, Japan, in 1996, produces two kinds of verotoxins, VT1 and VT2, encoded by the stx1 and stx2 genes. In the EHEC strains, as well as in other VT-producing E. coli strains, the toxins are encoded by lysogenic bacteriophages. The EHEC 0157:H7 strain RIMD 0509952 did not produce plaque-forming phage particles upon inducing treatments. We have determined the complete nucleotide sequence of a prophage, VT2-Sakai, carrying the stx2A and stx2B genes on the chromosome, and presumed the putative functions of the encoded proteins and the cis-acting DNA elements based on sequence homology data. To our surprise, the sequences in the regions of VT2-Sakai corresponding to the early gene regulators and replication proteins, and the DNA sequences recognized by the regulators share very limited homology to those of the VT2-encoding 933W phage carried by the EHEC 0157:H7 strain EDL933 reported by Plunkett et al. (J. Bacteriol., p1767-1778, 181, 1999), although the sequences corresponding to the structural components are almost identical. These data suggest that these two phages were derived from a common ancestral phage and that either or both of them underwent multiple genetic rearrangements. An IS629 insertion was found downstream of the stx2B gene and upstream of the lysis gene S, and this might be responsible for the absence of plaque-forming activity in the lysate obtained after inducing treatments.
UR - http://www.scopus.com/inward/record.url?scp=0033387903&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033387903&partnerID=8YFLogxK
U2 - 10.1266/ggs.74.227
DO - 10.1266/ggs.74.227
M3 - Article
C2 - 10734605
AN - SCOPUS:0033387903
VL - 74
SP - 227
EP - 239
JO - Genes and Genetic Systems
JF - Genes and Genetic Systems
SN - 1341-7568
IS - 5
ER -