TY - JOUR
T1 - Complete nucleotide sequences of 93-kb and 3.3-kb plasmids of an enterohemorrhagic Escherichia coli O157
T2 - H7 derived from Sakai outbreak
AU - Makino, Kozo
AU - Ishii, Kazuo
AU - Yasunaga, Teruo
AU - Hattori, Masahira
AU - Yokoyama, Katsushi
AU - Yutsudo, Chikako H.
AU - Kubota, Yoshino
AU - Yamaichi, Yoshiharu
AU - Iida, Tetsuya
AU - Yamamoto, Koichiro
AU - Honda, Takeshi
AU - Han, Chang Gyun
AU - Ohtsubo, Eiichi
AU - Kasamatsu, Masaaki
AU - Hayashi, Tetsuya
AU - Kuhara, Satoru
AU - Shinagawa, Hideo
PY - 1998
Y1 - 1998
N2 - Enterohemorrhagic Escherichia coli (EHEC) O157:H7, derived from an outbreak in Sakai city, Japan in 1996, possesses two kinds of plasmids: a 93-kb plasmid termed pO157, found in clinical EHEC isolates world-wide and a 3.3-kb plasmid termed pOSAK1, prevalent in EHEC strains isolated in Japan. Complete nucleotide sequences of both plasmids have been determined, and the putative functions of the encoded proteins and the cis-acting DNA sequences have been analyzed. pO157 shares strikingly similar genes and DNA sequences with F-factor and the transmissible drug-resistant plasmid R100 for DNA replication, copy number control, plasmid segregation, conjugative functions and stable maintenance in the host, although it is defective in DNA transfer by conjugation due to the truncation and deletion of the required genes and DNA sequences. In addition, it encodes several proteins implicated in EHEC pathogenicity such as an EHEC hemolysin (HlyA), a catalase-peroxidase (KatP), a serine protease (EspP) and type II secretion system. pOSAK1 possesses a ColE1-like replication system, and the DNA sequence is extremely similar to that of a drug-resistant plasmid, NTP16, derived from Salmonella typhimurium except that it lacks drug resistance transposons.
AB - Enterohemorrhagic Escherichia coli (EHEC) O157:H7, derived from an outbreak in Sakai city, Japan in 1996, possesses two kinds of plasmids: a 93-kb plasmid termed pO157, found in clinical EHEC isolates world-wide and a 3.3-kb plasmid termed pOSAK1, prevalent in EHEC strains isolated in Japan. Complete nucleotide sequences of both plasmids have been determined, and the putative functions of the encoded proteins and the cis-acting DNA sequences have been analyzed. pO157 shares strikingly similar genes and DNA sequences with F-factor and the transmissible drug-resistant plasmid R100 for DNA replication, copy number control, plasmid segregation, conjugative functions and stable maintenance in the host, although it is defective in DNA transfer by conjugation due to the truncation and deletion of the required genes and DNA sequences. In addition, it encodes several proteins implicated in EHEC pathogenicity such as an EHEC hemolysin (HlyA), a catalase-peroxidase (KatP), a serine protease (EspP) and type II secretion system. pOSAK1 possesses a ColE1-like replication system, and the DNA sequence is extremely similar to that of a drug-resistant plasmid, NTP16, derived from Salmonella typhimurium except that it lacks drug resistance transposons.
KW - Enterohemorrhagic Escherichia coli
KW - F-factor
KW - Nucleotide sequence
KW - Pathogenicity
KW - Plasmid
KW - pO157
KW - pOSAK1
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M3 - Article
C2 - 9628576
AN - SCOPUS:0032574019
SN - 1340-2838
VL - 5
SP - 1
EP - 9
JO - DNA Research
JF - DNA Research
IS - 1
ER -