Complete nucleotide sequences of 93-kb and 3.3-kb plasmids of an enterohemorrhagic Escherichia coli O157: H7 derived from Sakai outbreak

Kozo Makino, Kazuo Ishii, Teruo Yasunaga, Masahira Hattori, Katsushi Yokoyama, Chikako H. Yutsudo, Yoshino Kubota, Yoshiharu Yamaichi, Tetsuya Iida, Koichiro Yamamoto, Takeshi Honda, Chang Gyun Han, Eiichi Ohtsubo, Masaaki Kasamatsu, Tetsuya Hayashi, Satoru Kuhara, Hideo Shinagawa*

*この研究の対応する著者

研究成果: Article査読

155 被引用数 (Scopus)

抄録

Enterohemorrhagic Escherichia coli (EHEC) O157:H7, derived from an outbreak in Sakai city, Japan in 1996, possesses two kinds of plasmids: a 93-kb plasmid termed pO157, found in clinical EHEC isolates world-wide and a 3.3-kb plasmid termed pOSAK1, prevalent in EHEC strains isolated in Japan. Complete nucleotide sequences of both plasmids have been determined, and the putative functions of the encoded proteins and the cis-acting DNA sequences have been analyzed. pO157 shares strikingly similar genes and DNA sequences with F-factor and the transmissible drug-resistant plasmid R100 for DNA replication, copy number control, plasmid segregation, conjugative functions and stable maintenance in the host, although it is defective in DNA transfer by conjugation due to the truncation and deletion of the required genes and DNA sequences. In addition, it encodes several proteins implicated in EHEC pathogenicity such as an EHEC hemolysin (HlyA), a catalase-peroxidase (KatP), a serine protease (EspP) and type II secretion system. pOSAK1 possesses a ColE1-like replication system, and the DNA sequence is extremely similar to that of a drug-resistant plasmid, NTP16, derived from Salmonella typhimurium except that it lacks drug resistance transposons.

本文言語English
ページ(範囲)1-9
ページ数9
ジャーナルDNA Research
5
1
出版ステータスPublished - 1998
外部発表はい

ASJC Scopus subject areas

  • 遺伝学
  • 分子生物学

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