Comprehensive study of microgel electrode for on-chip electrophoretic cell sorting

研究成果: Article

11 引用 (Scopus)

抄録

We have developed an on-chip cell sorting system and microgel electrode for applying electrostatic force in microfluidic pathways in the chip. The advantages of agarose electrodes are 1) current-driven electrostatic force generation, 2) stability against pH change and chemicals, and 3) no bubble formation caused by electrolysis. We examined the carrier ion type and concentration dependence of microgel electrode impedance, and found that CoCl2 has less than 1/10 of the impedance from NaCl, and the reduction of the impedance of NaCl gel electrode was plateaued at 0.5 M. The structure control of the microgel electrode exploiting the surface tension of sol-state agarose was also introduced. The addition of 1% (w/v) trehalose into the microgel electrode allowed the frozen storage of the microgel electrode chip. The experimental results demonstrate the potential of our system and microgel electrode for practical applications in microfluidic chips.

元の言語English
ジャーナルJapanese Journal of Applied Physics
49
発行部数6 PART 2
DOI
出版物ステータスPublished - 2010 6
外部発表Yes

Fingerprint

classifying
Sorting
chips
Cells
Electrodes
electrodes
Electrostatic force
impedance
Microfluidics
electrostatics
Bubble formation
electrolysis
Sols
Electrolysis
Surface tension
interfacial tension
bubbles
Gels
gels
Ions

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

これを引用

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abstract = "We have developed an on-chip cell sorting system and microgel electrode for applying electrostatic force in microfluidic pathways in the chip. The advantages of agarose electrodes are 1) current-driven electrostatic force generation, 2) stability against pH change and chemicals, and 3) no bubble formation caused by electrolysis. We examined the carrier ion type and concentration dependence of microgel electrode impedance, and found that CoCl2 has less than 1/10 of the impedance from NaCl, and the reduction of the impedance of NaCl gel electrode was plateaued at 0.5 M. The structure control of the microgel electrode exploiting the surface tension of sol-state agarose was also introduced. The addition of 1{\%} (w/v) trehalose into the microgel electrode allowed the frozen storage of the microgel electrode chip. The experimental results demonstrate the potential of our system and microgel electrode for practical applications in microfluidic chips.",
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