Conclusive evidence of the reconstituted hexasome proven by native mass spectrometry

Nanako Azegami, Kazumi Saikusa, Yasuto Todokoro, Aritaka Nagadoi, Hitoshi Kurumizaka, Yoshifumi Nishimura*, Satoko Akashi

*この研究の対応する著者

    研究成果: Article査読

    19 被引用数 (Scopus)

    抄録

    It has been suggested that the hexasome, in which one of the H2A/H2B dimers is depleted from the canonical nucleosome core particle (NCP), is an essential intermediate during NCP assembly and disassembly, but little structural evidence of this exists. In this study, reconstituted products in a conventional NCP preparation were analyzed by native electrospray ionization mass spectrometry, and it was found that the hexasome, which migrated in a manner almost identical to that of the octasome NCP in native polyacrylamide gel electrophoresis, was produced simultaneously with the octasome NCP. This result might contribute to understanding the assembly and disassembly mechanism of NCPs.

    本文言語English
    ページ(範囲)5155-5157
    ページ数3
    ジャーナルBiochemistry
    52
    31
    DOI
    出版ステータスPublished - 2013 8月 6

    ASJC Scopus subject areas

    • 生化学

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