Conjugative gene transfer in marine cyanobacteria: Synechococcus sp., Synechocystis sp. and Pseudanabaena sp.

Koji Sode, Masahiro Tatara, Haruko Takeyama, J. Grant Burgess, Tadashi Matsunaga

研究成果: Article査読

41 被引用数 (Scopus)

抄録

Versatility of gene transfer by transconjugation in marine cyanobacteria was demonstrated. In this study, seven different marine cyanobacteria were used as recipient cells. First, transconjugation was carried out using the mobilizable transposon (Tn 5) carrying plasmid pSUP1021. Transconjugants were observed in all marine cyanobacteria tested. Second, the broad-host-range vector pKT230 (IncQ) was tested for transconjugation. pKT230 has been successfully transferred in a marine cyanobacterium Synechococcus sp. NKBG15041C, and replicated as an autonomous replicon without alteration in the restriction enzyme pattern. A maximum transfer efficiency of 5.2 × 10-4 transconjugants/recipient cell was observed, when mating was performed on agar plates containing low salinity (0.015 m NaCl) medium. This is the first study to demonstrate gene transfer in marine cyanobacteria via transconjugation.

本文言語English
ページ(範囲)369-373
ページ数5
ジャーナルApplied Microbiology and Biotechnology
37
3
DOI
出版ステータスPublished - 1992 6 1
外部発表はい

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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